西兰花茎叶多肽的酶法提取及其增强免疫力功能研究  

Immunomodulatory effects of the enzymatic extract polypeptide from broccoli stems and leaves

作　　者：党亚丽[1] 么春艳[1] 周亭屹 俞盈[1] 李青青[1] 吴庆其[1] 

机构地区：[1]浙江省医学科学院,浙江杭州310013

出　　处：《食品工业科技》2017年第11期352-355,共4页Science and Technology of Food Industry

基　　金：浙江省重大科技计划项目(2014C02023);浙江省医药卫生项目(201473497)

摘　　要：本文对西兰花茎叶多肽进行酶法提取以筛选增强免疫力功能活性强的组分。从西兰花茎叶中提取粗蛋白后,首先经酶法制备西兰花多肽,然后采用Valflow50超滤、Sephadex G-15进行分离纯化,得到分子量为310~2600 Da的西兰花小肽,并对其进行增强免疫力功能的动物实验研究,分别以100、50、25 mg/kg·bw 3个剂量的西兰花多肽提取物对小鼠连续灌胃30 d,然后分别测定小鼠的体液免疫功能、细胞免疫功能和单核-巨噬细胞功能。结果表明,高剂量组(100 mg/kg)西兰花多肽提取物对小鼠的淋巴细胞转化能力(0.225±0.040)与小鼠的左右耳片重量差异(18.19±2.94)mg均显著高于空白对照组(p<0.05)。西兰花多肽提取物对正常小鼠碳粒廓清功能有非常明显的促进作用,碳廓清指数分别为(0.126±0.052)和(0.136±0.022),高、中剂量能显著(p<0.05)增加小鼠非特异免疫功能。以上结果表明西兰花多肽提取物具有增强免疫力的功能,此研究可为西兰花的深度开发和综合利用提供基础数据和科学参考。The polypeptide extract was gained by enzymatic method from broccoli stems and leaves and its immunomodulatory effects had been studied.The crude protein was extracted from broccoli steins and leaves,and then the broccoli polypeptides were prepared by enzymatic methods. The hydrolysate was isolated by the method of ultrafihration and Sephadex G-15. The molecular mass of the oligopeptide was 310- 2600 Da. The animal experiments were studied on their immunity function. Oligopeptide were consecutively orally given to mice with the doses of 100,50,25 mg/kg.bw（ comparatively as 20,10,5 times of recommended human daily dosage） for 30 days.It was determinated for the humoral immune function, the cellular immune function and the mononuclear macrophage function in mice.Results showed that the high dose group （100 mg/kg）of broccoli peptide extracts had high conversion ability （0.225 ± 0.040 ）and the ear weight were different （18.19 ±2.94 ） mg, it was significantly higher than that of control group（p 〈0.05 ）.Broccoli polypeptide extracts have obvious effect on the normal mice carbon clearance function, carbon clearance index were （0.126 ± 0.052）and （0.136 ± 0.022 ）, high and medium dose significantly （p 〈 0.05 ）increased the nonspecific immune function of mice.Results showed that the broccoli polypeptide extract could enhance immunity function, which could provide basic data and the scientific reference for the deep development and comprehensive utilization of broccoli.

关 键 词：西兰花 多肽 提取 增强免疫力 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]