基于SiO_2荧光纳米粒子快速检测果汁中大肠杆菌的免疫传感器研究  

Biosensor for rapid detection of Escherichia coli in juice by luminophore-doped silica

作　　者：孙晓萌[1] 张志涛[1] 王轶鹏[1] 许强[1] 孙雪梅[1] 刘文敏[1] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062

出　　处：《食品安全质量检测学报》2017年第5期1824-1828,共5页Journal of Food Safety and Quality

摘　　要：目的设计一种基于SiO_2荧光纳米粒子快速检测果汁中大肠杆菌O157:H7的新型传感器。方法采用超分子组合法将荧光基团嵌入到SiO_2纳米颗粒中合成SiO_2荧光纳米粒子,然后将抗大肠杆菌O157:H7的单抗偶联到纳米粒子表面,最后通过抗原-抗体反应使纳米粒子与待检细菌结合,使用荧光显微镜观察并统计大肠杆菌O157:H7的个数。结果本方法与革兰氏染色法相比,对大肠杆菌O157:H7计数结果无统计学差异(P=0.930);该方法整个检测过程能够在15 min内完成,检测下限小于10 CFU/mL。结论该方法能增强检测果汁中大肠杆菌O157:H7的灵敏度,缩短检测时间,对其他病原体检测方法的改进具有重要的指导意义。Objective To design a new biosensor for rapid detection of Escherichia coli O157∶H7 in juice by synthesized luminophore-doped silica （LDS） nanoparticles.Methods The fluorescent groups were embed into SiO2 nanoparticles to synthesize LDS by supramolecular combination method.Then E.coli O 157∶H7 specific monoclonal antibody was coupled to the surface of the dye doped nanoparticles.Finally,antibody-coated narnoparticles incorporated with the cells of O157∶H7 by antigen-antibody reaction to form conglutinations,which represented the images of the detected germs and could be observed and counted by fluorescence microscope.Results The number of E.coli O157∶H7 was detected and counted with methods of fluorescent nanoparticles staining and gram staining,which had no significant difference （P=0.930）.The novel developed LDS nanoparticles could finish the detection within 15 mins,and had the limit of detection of 10 CFU/mL.Conclusion The method of LDS nanoparticles can enhance the sentivity of O157∶H7 detection and obviously shorten testing time,which has important significance for the improvement of inspection methods for other pathogenic microorganisms.

关 键 词：SiO2荧光纳米粒子 大肠杆菌O157:H7 快速检测 生物传感器 果汁 

分 类 号：O657.3[理学—分析化学] TS255.44[理学—化学]