纺锤形赖氨酸芽孢杆菌P-3产胞外多糖发酵工艺参数优化  

Optimization of fermentation technical parameters of Lysinibacillus fusiformis P-3 for exopolysaccharides production

作　　者：曾承露[1] 李锋[1] 黄德娜[1] 

机构地区：[1]黔南民族师范学院化学化工学院,贵州都匀558000

出　　处：《中国酿造》2017年第6期111-115,共5页China Brewing

基　　金：贵州省教育厅自然科学研究重点项目(黔教合KY[2014]286);贵州省科学技术联合基金项目(黔科合LH字[2014]7418)

摘　　要：利用响应面法对纺锤形赖氨酸芽孢杆菌(Lysinibacillus fusiformis)P-3产胞外多糖(EPS)发酵工艺参数进行了优化。通过单因素试验筛选到显著因素,对显著因素进行Box-Behnken中心组合试验设计优化,建立EPS产量与各因素的多元回归方程;最终确定最佳发酵工艺参数为接种量3%、初始pH 7、发酵温度34℃、发酵时间32 h和转速161 r/min。在此优化条件下,EPS平均产量为2.92 g/L。The fermentation technical parameters of exopolysaccharides （EPS）-producing Lysinibacillus fusiformis P-3 were optimized by response sur- face methodology. The significant factors were screened and optimized by single factor and Box-Behnken experiments. The multiple regression equa- tion of EPS yield and all the factors was established. Finally, the optimum fermentation technical parameters were determined as followed： inoculum 3%, initial pH 7, fermentation temperature 34 ℃, time 32 h and rotate speed 161 r/min. Under the optimal conditions, the average yield of EPS was 2.92 g/L.

关 键 词：纺锤形赖氨酸芽孢杆菌 胞外多糖 响应面法 发酵工艺 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]