葡萄中赭曲霉毒素产生真菌的筛选及侵染特性  

Screening and Infection Characteristics of Ochratoxin A Producing Fungi in Grapes

作　　者：雷纪锋 陈树兵 吴祖芳[1] 张鑫[1] 刘茜[1] 

机构地区：[1]应用海洋生物技术教育部重点实验室宁波大学海洋学院,浙江宁波315211 [2]宁波市出入境检验检疫局,浙江宁波315012

出　　处：《中国食品学报》2017年第5期186-193,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：宁波市自然科学基金项目(2014A610189)

摘　　要：为筛选葡萄中赭曲霉毒素产生菌及其侵染特性,以宁波地区的不同葡萄品种为材料,采用紫外荧光筛选结合液-质联用检测,对其OTA产生菌进行分离筛选。从葡萄中得到63株霉菌,主要由青霉属和曲霉属组成,其中8个菌株(包括5株青霉和3株曲霉)产荧光并产OTA,产毒能力均不强。将产毒能力最强的曲霉属菌株S_5于25℃下避光培养14 d,OTA相对含量仅8.14 ng/g。通过分析健康葡萄和损伤葡萄中OTA的含量及OTA产生菌的污染状况,发现相对于健康葡萄,损伤葡萄更易受OTA产生菌的污染,表明葡萄的损伤是造成OTA产生菌侵染并产生OTA的主要因素。本研究对葡萄及其制品的风险评估具有重要意义。In the present study, the isolating, screening and characteristics of Ochratoxin A （OTA） producing fungi in grapes were investigated. OTA producing fungi were isolated from different grapesin Ningbo area by ultraviolet fluorescence combining with LC-MS/MS. The results showed that 63 fungi obtained were mainly consisted by genus of Penicillium and Aspergillus, which 8 fungi（including 5 strains of Penicillium and 3 strains of AspergiUus） produced fluorescence and OTA. However, the contents of OTA they produced were relatively low. Strains of Aspergillus spp. （S5） produced the highest content of OTA for 8.14 ng/g when cultured at 25℃ with no light. In addition, the correlation of OTA content and OTA producing fungi in damaged and healthy grapes were also analyzed. It indicated that the damaged grapes are easily contaminated by OTA producing fungi compared with the healthy grapes. The results showed that the damage of grapes is a major factor of the infection of OTA producing fungiand OTA formation. Together, these results showed great significance to evaluate the risk of grapes and grape products.

关 键 词：赭曲霉毒素A 葡萄 紫外荧光法 筛选 

分 类 号：TS201.3[轻工技术与工程—食品科学]