离子液体超声辅助提取桑葚中原花青素  

Ionic Liquid Based Ultrasonic-assisted Extraction of Procyanidins from Mulberry

作　　者：张喜峰[1,2] 何倩[1] 张斌[1] 王明卉 罗光宏[3] 

机构地区：[1]河西学院农业与生物技术学院 [2]甘肃省河西走廊特色资源利用重点实验室 [3]河西学院凯源生物技术开发中心甘肃省微藻工程技术研究中心,张掖734000

出　　处：《天然产物研究与开发》2017年第6期963-970,共8页Natural Product Research and Development

基　　金：甘肃省中小企业创新基金(1047GCCG001);河西学院科研创新与应用校长基金(XZ2014-29);国家级大学生创新创业训练计划(201510740005);甘肃省河西走廊特色资源利用省级重点实验室资助(XZ1607)

摘　　要：采用离子液体超声辅助提取桑葚中原花青素,在单因素实验基础上,采用响应面优化[Bmim]Cl(1-丁基-3-甲基咪唑氯)浓度、料液比、超声功率、超声时间对桑葚中原花青素得率的影响,利用扫描电镜分析了该技术提取效果较好的原因。结果表明,桑葚中原花青素的最佳提取工艺为:[Bmim]Cl浓度为0.73 mol/L、料液比1∶21(g/mL)、超声功率188 W、超声时间11 min,此条件下原花青素得率可达3.51%,与模型预测值接近。扫描电镜观察发现,离子液体超声对桑葚粉末微观结构破坏影响较大。An ultrasonic-assisted extraction method was developed for the extraction of procyanidins from Mulberry with ionic liquid solution as extracting agent. The extraction parameters （ [ Bmim ] C1 concentration, solid to solvent ratio, ul- trasonic power, ultrasonic time） were optimized by single-factor method and response surface methodology based on Box- Behnken design. The reasons for high efficiency of this extraction technology were analyzed by scanning electron micro- scope （SEM）. The optimal extraction conditions were： the concentration of [ Bmim C1 of 0.73 mol/L, solid to solvent ratio of 1：21 （g/mL） , ultrasonic power of 188 W, and ultrasonic time of 11 min. The extraction yield of procyanidins was 3.51% ,which was well in close agreement with the value predicted by the model. The microcosmic structures of powders of Mulberry after being extracted by ionic liquid based ultrasonic-assisted approach were seriously destroyed from SEM analysis.

关 键 词：离子液体 原花青素 桑葚 扫描电镜 

分 类 号：TS201.2[轻工技术与工程—食品科学]