凝胶过滤色谱分离纯化玉米蛋白酶解产物研究  

Study on separation and purification of enzymatic hydrolysate of corn protein by gel filtration chromatography

作　　者：胡二坤[1] 郭兴凤[2] 刘洁[2,3] 

机构地区：[1]河南职业技术学院,河南郑州450000 [2]河南工业大学粮油食品学院,河南郑州450001 [3]河南经济贸易高级技工学校,河南新乡453000

出　　处：《粮食与油脂》2017年第7期94-98,共5页Cereals & Oils

基　　金：河南职业技术学院2017年度科研基金资助计划(2017-HZK-03)

摘　　要：研究了凝胶过滤色谱的分离条件如洗脱速度、待分离样品的浓度和待分离样品的体积对玉米蛋白酶解产物分离纯化的影响,确定了凝胶过滤色谱分离纯化玉米蛋白酶解产物的最佳试验条件。同时进一步研究了玉米蛋白酶解产物的分子量与其抗氧化活性之间的关系,研究表明,玉米蛋白酶解产物的分子量与抗氧化能力之间具有相关性,即分子量越小,抗氧化能力越强。The effects of elution rate, sample concentration and sample volume on the separation and purification of corn protein hydrolysates were studied. The optimum of gel filtration chromatography conditions were selected. At the same time, the relationship between the molecular size and the antioxidant activity of the hydrolysates was studied. The results showed that there was a correlation between the molecular size of corn protein hydrolysates and antioxidant capacity. Smaller molecule weight was, stronger antioxidant capacity was.

关 键 词：凝胶过滤色谱 蛋白水解物 分离纯化 

分 类 号：TS201.2[轻工技术与工程—食品科学]