2013年夏季北京市售整鸡沙门菌分离株耐药性及耐药机制研究  

Antimicrobial resistance analysis and mechanism of Salmonella recovered from retail chicken carcasses in Beijing in summer,2013

作　　者：胡豫杰[1] 赫英英 白瑶[1] 李凤琴[1] 徐进[1] 

机构地区：[1]国家食品安全风险评估中心卫生部食品安全风险评估重点实验室,北京100021 [2]北京市海淀区疾病预防控制中心

出　　处：《卫生研究》2017年第4期538-545,共8页Journal of Hygiene Research

基　　金：国家食品安全风险评估中心高层次人才队伍建设523项目(2017人才-3-11;2017人才-3-4)

摘　　要：目的了解2013年夏季北京市售整鸡样品中分离的166株沙门菌耐药性及耐药机制。方法采用微量肉汤稀释法对166株沙门菌进行9类11种抗生素的耐药性检测,挑选头孢类耐药株进行超广谱β-内酰胺酶(ESBLs)检测,并对环丙沙星和头孢噻肟双耐药株进行耐药机制分析。结果 94.6%(157/166)的沙门菌至少对一种抗生素耐药,多重耐药株占58.4%,对萘啶酸耐药率最高,为92.2%(153/166),其他依次为氨苄西林(48.8%)、氨苄西林-舒巴坦(44.0%)和四环素(44.0%)。共有27种耐药谱,常见的耐药谱为萘啶酸(n=46,29.3%)、萘啶酸-氨苄西林-氨苄西林/舒巴坦(n=28,17.8%)。47株头孢噻肟耐药株中有41株(87.2%)ESBLs阳性,其中有38株同时对环丙沙星耐药。这38株环丙沙星和头孢噻肟双耐药且ESBLs阳性的沙门菌中存在喹诺酮耐药决定区(QRDRs)、质粒介导喹诺酮耐药(PMQR)和ESBLs等耐药机制,37株测试菌株的GyrA的第83位、87位氨基酸和ParC的57位、80位氨基酸存在不同程度突变。38株测试菌株均检测到不同种类和数量的PMQR基因,如qnrB、qnrS、oqxAB和aac(6’)-Ib-cr等,部分基因存在突变。主要存在的β-内酰胺酶类型为CTX-M型(35株)、TEM型(20株)和OXA型(36株),未检测到SHV型和AmpC型。结论北京市售整鸡中沙门菌整体耐药水平较严重,需强化对肉鸡养殖、屠宰、运输、销售等环节中沙门菌耐药性监测,分析耐药机理及传递机制,同时注意临床感染治疗用药监管。Objective Research were carried out to determine the antimicrobial resistance and mechanism of Salmonella isolates recovered from retail chicken carcasses in Beijing in summer, 2013. Methods Broth microdilution method were used for antimicrobial susceptibility testing to obtain the minimal inhibitory concentration（ MICs）against 11 antimicrobial compounds which belongs to 8 categories and 166 Salmonella strains isolated from 33 retail chicken carcasses in Beijing in summer,2013 were tested.Cephalosporin resistant isolates were evaluated by ESBLs confirmation test,also resistance mechanism analysis was subjected to ciprofloxacin and cefotaxime co-resistant Salmonella isolates. Results 94. 6% isolates were resistant to at least one antimicrobial and 58. 4% were identified as multi-drug resistant strains. The highest rate of resistance was 92. 2% against nalidixic acid, followed by ampicillin（ 48. 8%）, ampicillin-sulbactam（ 44. 0%）,tetracycline（ 44. 0%）. There were 27 antimicrobial resistance spectrums with NAL and NAL-AMP-SAM being dominant spectrums. Totally,41 ESBLs positive strains which contained 38 ciprofloxacin resistant isolates were recovered from 47 cefotaxime resistant strains. Detection of quinolones and β-lactams resistant genes for 38 ciprofloxacin and cefotaxime co-resistant and ESBLs positive Salmonella isolates indicate that QRDRs/PMQR/ESBLs antimicrobial resistance mechanism existed in these strains. Point mutations in gyrA and parC were identified in 37 isolates,and 38 isolates tested all contained PMQR genes such as qnrB、qnrS、oqxAB and aac（ 6 ＇）-Ib-cr genes with a few mutations,while CTX-M（ n = 35）,TEM（ n = 20）,OXA（ n = 36） type β-lactamases were detected.Conclusion High level antimicrobial resistance and complicated mechanism existed among Salmonella recovered from retail chicken carcasses in Beijing in summer,2013. In order to protect public health,tendency and further mechanism on antimicrobial resistance and transmissibility should be described deeply by

关 键 词：市售整鸡 沙门菌 耐药性 耐药机制 

分 类 号：R155.55[医药卫生—营养与食品卫生学] TS207.4[医药卫生—公共卫生与预防医学]