实时定量荧光PCR快速鉴定食品中单核细胞增生李斯特氏菌  

Rapid detection of Listeria monocytogenes in food by real-time PCR

作　　者：刘万静 刘斌[2] 李湘平[2] 李海燕[2] 石红[1] 吴友伟[1] 答嵘[1] 

机构地区：[1]西安交通大学第一附属医院检验科,西安710061 [2]安康市疾病预防控制中心生物检验科,安康725000

出　　处：《食品安全质量检测学报》2017年第1期252-255,共4页Journal of Food Safety and Quality

摘　　要：目的建立实时定量荧光PCR法(real-time PCR)快速鉴定食品中的单核细胞增生李斯特氏菌(Listeria monocytogenes,LM)。方法选取2016年国家食品风险监测样本134例与模拟灭活LM样本10例,采用GB/T4789.30-2010与real-time PCR方法同步检测单核细胞增生李斯特菌。结果共检测食品134份,包括肉制品、水产品、快餐和即食食品等。共检出8株LM,检出率为5.97%。以GB/T4789.30-2010为金标准判断,real-time PCR方法检测样本中LM的灵敏度与特异度均达到100%。模拟灭活LM样本real-time PCR方法检出率为100%,标准法检出率为0%。结论本方法可以简化实验程序,减少工作量,节约检测试剂,为可能发生的食物中毒尽早提供实验依据。Objective To establish the method for rapid identification of Listeria monocytogenes（LM） in food by real-time PCR. Methods One hundred and thirty-four cases of national food risk monitoring samples in 2016 and 10 cases of simulated inactivated LM samples were simultaneously detected by GB/T4789.30-2010 method and real-time PCR method. Results A total of 134 samples of food including meat products, aquatic products, fast food and instant food etc. were enrolled. Totally 8 strains of LM were detected and the detection rate was 5.97 %. Using GB/T4789.30-2010 as the gold standard, the sensitivity and specificity of real-time PCR were both up to 100%. In 10 samples of simulated inactivated LM, the detection rate of real-time PCR method was 100%, but that of the standard method was 0%. Conclusion The established real-time PCR method can simplify the experiment procedure, reduce the workload and save the reagent, which can provide experimental basis for probable food poisoning as early as possible.

关 键 词：单核细胞增生李斯特氏菌 实时定量荧光PCR 食品 

分 类 号：TS207.4[轻工技术与工程—食品科学]