酶法辅助提取猴头菇中硒多糖工艺中复合酶用量的优化  

The Optimization of Dosage of Complex Enzymes in Enzyme-assisted Hericium Se-polysaccharides Extraction

作　　者：顾浩峰[1] 

机构地区：[1]安康学院现代农业与生物科技学院,陕西安康725000

出　　处：《食品与发酵科技》2017年第4期39-44,共6页Food and Fermentation Science & Technology

基　　金：陕西省农业科技创新与攻关项目(2015NY105);陕西省教育厅专项科研项目(15JK1003);安康学院大学生创新创业项目(2016akxy018)

摘　　要：以安康富硒猴头菇为原料,运用单因素和正交试验设计,对酶法辅助提取猴头菇硒多糖工艺中复合酶(纤维素酶、果胶酶和木瓜蛋白酶)的添加量进行优化。结果表明:各酶的最优添加量为纤维素酶0.5%,果胶酶0.6%,木瓜蛋白酶0.4%。在酶的最优添加量下,硒多糖的提取率可达17.52%,硒多糖中硒含量为0.282mg/kg。Se-enriched Hericium grown in Ankang was used as the raw material, andadditive amount of complex enzymes （cellulase, pectinase and papain） in enzymes-assisted se-polysaceharides extracting process was optimized by single factor test and orthogonal test, with the extraction rate of se-polysaccharides as indicator. The results in- dicated that the optimal dosage of each enzyme in se-polysaccharides extracting process was as follows, cellulase 0.5%, pectinase 0.6%, papain 0.4%. Based on the optimal condition, the extraction rate of se-polysaccharides was reach 17.52%,and the content of selenium was 0.282mg/kg.

关 键 词：酶法辅助提取 猴头菇 正交试验 硒多糖 

分 类 号：TQ925.5[轻工技术与工程—发酵工程]