酒酒球菌β-葡萄糖苷酶基因克隆及生物信息学分析  

Cloning and Bioinformatic Analysis of the β-glucosidase Gene in Oenococcus oeni

作　　者：杨世玲[1] 刘叶[1] 薛楚然 王玲[1] 何玲[2] 刘树文[1,3,4] 

机构地区：[1]西北农林科技大学葡萄酒学院,陕西杨凌712100 [2]西北农林科技大学园艺学院,陕西杨凌712100 [3]陕西省葡萄与葡萄酒工程技术研究中心,陕西杨凌712100 [4]西北农林科技大学合阳葡萄试验示范站,陕西渭南715300

出　　处：《中国食品学报》2017年第7期199-207,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2016YFD0400504);国家现代农业产业技术体系建设专项(nycytx-30-ch-03)

摘　　要：【目的】从分子水平上揭示酒酒球菌β-葡萄糖苷酶基因(bgl)的结构特点,为β-葡萄糖苷在酒酒球菌中的代谢研究提供理论依据。【方法】采用PCR法从4株优良的酒酒球菌(SD-1d,SD-2gf,31-DH和NM-2c)中克隆目的基因,并对其编码产物进行生物信息学分析。【结果】bgl基因含有一个完整的开放阅读框,全长2 215 bp,编码737个氨基酸,起始密码子为TTG,终止密码子为TAA。生物信息学分析均显示该蛋白疏水性较弱,无信号肽,无跨膜区,属于糖苷水解酶家族3成员。【结论】bgl基因编码产物为1个81 625.3 u蛋白,具有一个明显的β-葡萄糖苷酶活性位点,为非分泌蛋白,主要存在于细胞质中。[Objective]The aim of the work is to reveal the construction features of β-glucosidase gene（bgl） in Oenococ cus oeni in the molecular level and to provide a theoretical direction for further exploring the β-glucosides metabolism in O. oeni cells. [Methods] The target gene was cloned by PCR from the genomic DNA of four O. oeni strains（SD-1d, SD-2gf, 31-DH and NM-2c）, and the encoded productions were analyzed by bioinformatics technology.[Results] The results showed that the bgl gene has a completely open reading frame, being 2215 bp and encoding 737 amino acids. The initiation codon is TTG and the termination codon is TAA. Bioinformatic analysis indicated that the encoded protein was of weak hydrophobicity, no signal peptide and transmembrane helice, stabilization and heat-resistance,belonging to the superfamily of Glycohydro3. [Conclusion] The encoded productions of bgl gene were a protein of81625.3 u and showed BGL activity. It was considered to be non-secretory cytoplasmic protein and located in cytoplasm.

关 键 词：酒酒球菌 Β-葡萄糖苷酶 基因克隆 生物信息学分析 

分 类 号：Q811.4[生物学—生物工程] TS262.6[轻工技术与工程—发酵工程]