灵芝液体发酵产胞外多糖培养基的优化  

Optimization of Fermentation Medium for Production of Extracellular Polysaccharide from Ganoderma lucidum

作　　者：钱磊[1] 张志军[1] 陈晓明[1] 李淑芳[1] 李凤美[1] 刘建华[1] 

机构地区：[1]天津市林业果树研究所,天津300384

出　　处：《食品研究与开发》2017年第17期10-13,共4页Food Research and Development

基　　金：天津市农业科学院院长基金(16009);天津市林业果树研究所所长基金(SZJJ2016005);天津市科技重大专项与工程计划项目(15ZXBFNC00250)

摘　　要：采用摇瓶液体发酵法探讨了培养基组成对灵芝胞外多糖产量的影响。单因素和正交试验结果表明,摇瓶发酵培养基的最佳配方为豆饼粉0.2%、蔗糖2%、KH_2PO_4 0.1%、FeSO_4 0.05%、VB10.005%,其中豆饼粉和蔗糖为显著性因素。在此条件下,胞外多糖产量达213.9 mg/100 mL。The effects of medium composition on the production of extracellular polysaccharide from Ganoderma lucidum were studied by shake flask liquid fermentation. The results of single factor and orthogonal test showed that the optimal formula of fermentation medium was soybean powder 0.2 %,sucrose 2 %,KH_2PO_4 0.1 %,FeSO_4 0.05 % and VB10.005 %,in which soybean powder and sucrose were significant factors. Under these conditions,the yield of extracellular polysaccharide reached 213.9 mg/100 mL.

关 键 词：灵芝 胞外多糖 培养基 优化 

分 类 号：TQ929[轻工技术与工程—发酵工程]