百香果籽油超临界CO_2萃取工艺优化及其体外抗氧化活性  

Optimization of supercritical CO_2 extraction for passion fruit seed oil by response surface methodology and investigation of antioxidant activities of extracted oil in vitro

作　　者：马金魁[1] 李珂[2] 韦炳墩 姚紫婷 黄晓辰[3] 

机构地区：[1]肇庆学院化学化工学院,广东肇庆526061 [2]湖南农业大学食品科技学院,湖南长沙410128 [3]肇庆学院生命科学学院,广东肇庆526061

出　　处：《食品与机械》2017年第7期155-159,共5页Food and Machinery

基　　金：广东省青年创新人才项目(编号:2016KQNCX176;2015KQNCX182);广东大学生科技创新培育专项资金(编号:pdjh2017b0552;pdjh2017b0550);大学生创新创业训练计划项目(编号:201610580057)

摘　　要：以百香果为原料,在单因素试验基础上,采用Box-Behnken响应面分析,优化超临界CO_2萃取百香果籽油的工艺,并对百香果籽油的体外抗氧化活性进行研究。结果表明,超临界CO_2萃取百香果籽油的最佳工艺为萃取温度53.1℃,萃取压力33.9 MPa,萃取时间3.6h,百香果籽出油率值为26.95%,所得百香果籽油具有较好的还原力,且呈量效关系,对DPPH·的清除能力达80%。超临界CO_2萃取百香果籽油工艺稳定可行,提取的百香果籽油具有抗氧化活性,是一种潜在可用的天然抗氧化资源。The Box-Behnken design and response surface methodology（RSM）were employed to optimize the process parameters of supercritical carbon dioxide extraction of the passion fruit seed oil and the antioxidant activities of the extracts was also investigated in this study.Results obtained from the RSM showed that the optimum extraction process parameters within the experimental ranges would be the extraction temperature of 53.1 ℃, pressure of33.9 MPa,and extraction time of 3.6h.Under these conditions,the oil yield was 26.95%.The results of the antioxidant experimental also showed that the extracted oil have a potent reducing power and the relative antioxidant ability of oil to scavenge the DPPH·radicals was found to be 80%.Based on these results it can be concluded that supercritical carbon dioxide extraction could be applied to the oil extraction of the passion fruit and the extracted oil have shown promise as a natural antioxidant resource.

关 键 词：百香果 籽油 超临界CO2萃取 抗氧化性 

分 类 号：TS209[轻工技术与工程—食品科学]