酸水解-柱前衍生化-HPLC-MS/MS法分析三种即食海产品中的酸性多糖  

Analysis of acidic polysaccharides of 3 types of ready-to-eat seafood products using PMP-HPLC-MS/MS after acid hydrolysis

作　　者：徐鑫[1] 鲁姣姣[1] 刘海曼 张豹[1] 武苏凤 宋爽[1,2,3] 

机构地区：[1]大连工业大学食品学院,大连116034 [2]国家海洋食品工程技术研究中心,大连116034 [3]海洋活性多糖开发应用技术国家地方联合工程实验室,大连116034

出　　处：《分析试验室》2017年第9期1053-1057,共5页Chinese Journal of Analysis Laboratory

基　　金：海洋公益性行业科研专项经费(201505029);大连市支持高层次人才创新创业项目(2016RQ061)资助

摘　　要：从扇贝丁、鱿鱼丝和鳕鱼片中提取得到粗多糖,使用1.3 mol/L的三氟乙酸(TFA)进行降解,1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化后,高效液相色谱-三重四级杆质谱(HPLC-MS/MS)分析降解产物中的二糖片段。扇贝丁和鱿鱼丝中的酸性多糖几乎全部为硫酸软骨素;而鳕鱼片不仅含有硫酸软骨素,还含有少量透明质酸,相对含量分别为87.5%和12.5%。Sallop adductor,shredded squid tube,and cod fillet are 3 popular ready-to-eat seafood products, and their acidic polysaccharides are important nutrient components. In this study,polysaccharides were prepared from three ready-to-eat seafood products and then hydrolyzed by 1. 3 mol/L trifluoroacetic acid（ TFA）. After derivatization with 1-phenyl-3-methyl-5-pyrazolone（ PMP）, the disaccharides were analyzed by high performance liquid chromatography-triple quadrupole mass spectrometer（ HPLC-MS/MS）. As indicated by the experiment results,chondroitin sulfate（ CS） was almost the only acidic polysaccharide observed in sallop adductor and shredded squid tube,while cod fillet contains both CS and hyaluronic acid（ HA） with relative contents of 87. 5% and 12. 5%,respectively.

关 键 词：海产品 酸性多糖 硫酸软骨素 透明质酸 

分 类 号：TS201.6[轻工技术与工程—食品科学]