以菊粉为底物全细胞催化生产甘露醇  

The whole cell catalytic production of mannitol using inulin as substrate

作　　者：罗希[1] 曹海龙[2] 张卉妍 李悝悝 张春枝[1] LUO Xi CAO Hailong ZHANG Huiyan LI Kuikui ZHANG Chunzhi(School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China)

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]中国科学院大连化学物理研究所,辽宁大连116023

出　　处：《大连工业大学学报》2017年第4期235-239,共5页Journal of Dalian Polytechnic University

基　　金：国家高技术研究发展计划(863计划)项目(2014AA093511)

摘　　要：将Leuconostoc mesenteroides来源的甘露醇脱氢酶(mdh)及Mycobacterium vaccae来源的甲酸脱氢酶(fdh)的共表达载体pRSFDuet-mdh-fdh,与葡萄糖辅助蛋白(glf)的表达载体pZY507glf共转入大肠杆菌BL21(DE3)中,成功构建了一个可将果糖转化为甘露醇的全细胞催化剂E.coli BL21(DE3)/pRSFDuet-mdh-fdh pZY507glf。该全细胞催化剂在pH 6.5、温度30℃时,具有最高的转化效率,甘露醇的产率可达到0.91g/g。通过与高活力的菊粉酶协同作用,甘露醇的产率可达到0.93g/g。A whole-cell biotransformation system E. coli BL21 （DE3）/pRSFDuet-mdh-fdh pZY507glf for mannitol production were established through the mannitol dehydrogenase （mdh） derived from Leuconostoc rnesenteroides and the formate dehydrogenase gene （fdh） derived from Mycobacteriurn vaccae pRSFDuet-mdh-fdh with the glucose facilitator protein （glf） pZY507glf co-expressed in E. coli BL21（DE3）. The yield of mannitol could reach to 0.91 g/g under the optimum conditions of pH 6.5 at 30 ℃. The yield of mannitol could reach to 0.93 g/g by whole catalyst with high activity inulinase.

关 键 词：甘露醇 生物转化法 菊粉 全细胞催化 

分 类 号：TS202.1[轻工技术与工程—食品科学] Q819[轻工技术与工程—食品科学与工程]