大豆皂苷Ⅰ与Ⅱ及苷元B对黄曲霉毒素B1和苯并芘突变效应的拮抗  

Protective Activities of Soyasaponin Ⅰ,Ⅱ and Soyasapogenol B Against Aflatoxin B1 and Benzo[a] Pyrene-Induced Mutagenicity

作　　者：光翠娥 干建平[1] 杨红飞[2] 李志刚[2] 

机构地区：[1]黄冈师范学院经济林木种质改良与资源综合利用湖北省重点实验室/大别山特色资源开发湖北省协同创新中心,湖北黄冈438000 [2]食品科学与技术国家重点实验室,江南大学,江苏无锡214122

出　　处：《食品与生物技术学报》2017年第8期814-818,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31201289);经济林木种质改良与资源综合利用湖北省重点实验室&大别山特色资源开发湖北省协同创新中心联合开放基金项目(2017AW01)

摘　　要：本研究确认了大豆皂苷Ⅰ与Ⅱ及苷元B对黄曲霉毒素B1和苯并芘的抗突变作用。Ames试验中,除了最小剂量时大豆皂苷Ⅰ与Ⅱ未能显著抑制移码突变外,3个受试物均明显抑制黄曲霉毒素B1和苯并芘诱发的TA98和TA100回复突变。而且,除了最小剂量时大豆皂苷Ⅰ与Ⅱ未能显著抑制苯并芘与DNA的结合外,3个受试物均明显抑制人肝癌(HepG2)及支气管上皮(BEAS-2B)细胞中突变剂与DNA加合物的形成。大体上,苷元B比大豆皂苷Ⅰ与Ⅱ更有效地抑制移码与碱基置换突变和加合物的形成。因此,3个食源活性成分不仅抑制基因突变同时也拮抗突变剂诱导的DNA损害。Inhibitory activities of soyasaponin I ,II and their aglycone for aflatoxin B 1 （AFB1） and benzo[ a]pyrene （B [ a]P ） -induced mutagenicity were investigated. In the Ames assay, Salmonella typhimurium strains TA98 and TA100 were selected for frameshift and base substitution mutations, respectively. Three compounds showed remarkable protection against AFB1 and B [ a] P-induced mutations,with the exception that at the low concentration of 0.3 mg/plate soyasaponins I and II did not significantly inhibit the frameshift mutation. Three compounds also effectively protected against the formation of AFB1-DNA adduct in HepG2 cells and B [ a] P-DNA adduct in BEAS-2B cells, with the exception that at the low concentration of 5 g/ml soyasaponins I and II showed no significant inhibition for the binding of B [ a]P to DNA. Generally, soyasapogenol B more effectively suppressed AFB1 and B [ a]P-induced frameshift and base substitution mutations and showed stronger inhibitory activities in the formation of adducts than soyasaponins I and II. These results indicate that three bioactives not only inhibit gene mutations but also protect against mutagen-induced DNA damage.

关 键 词：大豆皂苷 黄曲霉毒素 苯并芘 AMES试验 DNA加合物 

分 类 号：R99[医药卫生—毒理学]