固定化酶辊多效酶解大豆胚片的工艺优化  

Optimization of Sequential Hydrolysis of Soybean Flakes by Immobilized Enzyme Rollers for Increased Cold-Presssed Oil Yield

作　　者：李中宾 任悦[1] 邹德智[1] 王旭[1] 于殿宇[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品科学》2017年第22期67-73,共7页Food Science

基　　金："十三五"国家重点研发计划重点专项(2016YD0401402);国家自然科学基金面上项目(31571880)

摘　　要：利用醋酸纤维素修饰聚丙烯微孔膜,再将纤维素酶和中性蛋白酶分别固定在醋酸纤维素/聚丙烯复合膜上,并制成固定化酶辊,以大豆胚片为原料,依次按纤维素酶辊、中性蛋白酶辊的顺序共同交替多效循环对其进行碾压酶解,以冷榨大豆胚片的出油率为指标,在单因素试验的基础上,采用响应面法优化固定化酶辊酶解大豆胚片条件。结果表明,最佳条件为酶解环境空气相对湿度86.0%、大豆料胚温度55.0℃、酶辊碾压时间91.0 min,在此条件下酶解的大豆胚片经冷榨出油率为73.8%,瓦饼的蛋白变性较小,2种固定化酶辊重复使用7次后,其相对酶活力仍保持在80%以上。An immobilized enzyme roller was developed by immobilizing cellulase and neutral protease onto polypropylene microporous membrane modified by cellulose acetate, respectively. Soybean flakes were rolled sequentially with the cellulose roller followed by the neutral protease roller and then used for the production of cold-pressed oil. Hydrolysis conditions were optimized by one-factor-at-a-time method and response surface methodology. An air relative humidity of 86.0%, an operating temperature of 55.0 ℃ and a rolling time of 91.0 min were found to be the optimal conditions to obtain a higher oil yield of 73.8% and a lower degree of protein denaturation. After seventh repeated use, both immobilized enzyme rollers retained more than 80% of their initial activities.

关 键 词：固定化酶辊 多效酶解 冷榨法 出油率 氮溶解指数(NSI) 

分 类 号：TS224.3[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ644.1[轻工技术与工程—食品科学与工程]