超声波辅助复合酶法制备藏系绵羊胎盘肽的工艺优化  

Optimization of preparation of Tibetan sheep placenta peptide by ultrasonic-assisted composite enzymatic hydrolysis

作　　者：张丙云[1] 王聪[1] 谢言言 任海伟[1] 范文广[1] 黄思瑶 李志忠[1] 陈伟 ZHANG Bing-yun WANG Cong XIE Yan-yan REN Hai-wei FAN Wen-guang HUANG Si-yao LI Zhi-zhong CHEN Wei(School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China Lanzhou Mingde Agriculture-husbandry Technology Co. ,Ltd. ,Lanzhou 730010, China)

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]兰州名德农牧科技有限公司,甘肃兰州730010

出　　处：《食品工业科技》2017年第21期130-136,共7页Science and Technology of Food Industry

基　　金：甘肃省自然科学基金(1606RJZA206;1606RJYA287);兰州市科技计划项目(2014-2-20);兰州理工大学红柳青年教师培养计划(Q201207)

摘　　要：为实现羊胎盘深度开发利用,以肽得率和水解度为指标,筛选确定多种蛋白酶复合水解的最佳组合方案,并利用响应面法优化超声波辅助复合酶法水解制备羊胎盘肽的预处理参数。结果表明,响应面法优化后的超声预处理参数为超声时间18 min、温度28℃和功率420 W,超声波预处理后羊胎盘经木瓜蛋白酶和风味酶联合同步复合酶解制备羊胎盘肽的肽得率为39.89%,水解度为21.78%;该最优条件下平均肽链长度为4.59,分子量约500~600。结论:超声波辅助复合酶法制备藏系绵羊胎盘肽的工艺可行且有效。In order to realize the development and utilization of sheep placenta, the optimal combination scheme for the composite enzymatic hydrolysis of multiple proteases was investigated with the indicators of the yield of peptide （PY） and the degree of hydrolysis （DH）.On this basis, the ultrasonic pretreatment parameters of sheep placenta were optimized by response surface methodology（ RSM）.The results showed that the optimal conditions of ultrasound pretreatment were found as follows： ultrasonic time of 18 min,temperature of 28 ~C and power of 420 W.Under these optimal ultrasound pretreatment conditions, synchronous composite enzymatic preparation by papain and flavor enzyme of the PY was 39.89%, the DH was 21.78% .And the average chain length was 4.59 and the molecular weight was about 500 ~ 600. Conclusion ： the ultrasonic assisted enzymatic preparation method was feasible and effective in the preparation of Tibetan sheep placenta peptide.

关 键 词：藏系绵羊 羊胎盘肽 木瓜蛋白酶 风味酶 超声波预处理 工艺优化 

分 类 号：TS251.95[轻工技术与工程—农产品加工及贮藏工程]