鲢鱼鱼精蛋白超声波辅助提取工艺优化及其抗菌活性研究  

Optimization of extraction process assisted by ultrasonic and antibacterial activity evaluation on the protamine from silver carp

作　　者：刘光宪[1,2] 祝水兰[1] 何家林[1] 周巾英[1] 冯健雄[1] LIU Guang-xian;ZHU Shui-lan;HE Jia-lin;ZHOU Jin-ying;FENG Jian-xiong(Jiangxi Academy of Agricultural Sciences, Nanchang , Jiangxi 330200, China;Jiangxi Normal University, Nanchang, Jiangxi 330022, China)

机构地区：[1]江西省农业科学院,江西南昌330200 [2]江西师范大学,江西南昌330022

出　　处：《食品与机械》2017年第9期151-155,共5页Food and Machinery

基　　金：江西省农业科学院青年基金(编号:2015CQN002);江西省科技计划(编号:20161BBF60134)

摘　　要：为了实现鲢鱼加工副产物的高效综合利用,以鲢鱼精巢为原料,研究柠檬酸浓度、提取温度、超声功率、提取时间、固液比、提取次数对鱼精蛋白得率的影响。在单因素试验基础上,通过正交试验获得最佳提取工艺为:柠檬酸浓度0.45mol/L,温度55℃,超声时间20min,超声功率400 W,固液比130(g/mL),提取3次。在最佳工艺条件下,鱼精蛋白得率为3.25%;鱼精粗蛋白经Sepharose Fast Flow离子交换层析分离得到4个组分,对4个组分进行抗菌性分析可知,除鱼精蛋白的前体蛋白外,其他组分对沙门氏菌、金黄色葡萄球菌和大肠杆菌均具有较好的抑制活性,其中组分II、III的抑菌活性较好。To realize the high comprehensive utilization on the byproduct of silver carp,taking the testis sacs of silver carp as the raw material,the effects were studied,including the concentration of citric acid,the temperature,ultrasonic power,ultrasonic time,solid-liquid rate and extraction times on the efficacy extraction of silver carp spermary.On the base of single-factor experiments,and through the orthogonal test,the optimization experiment conditions were as followed：concentration of citric acid 0.45 mol/L,extraction temperature 55 ℃,the ultrasonic time 20 min,ultrasonic power400 W,ratio of solid-liquid 130,and the extraction times 3 times.The extraction ratio of chub protamine was 3.25% at the optimized conditions.Four groups chub protamine were purified and separated through sepharose fast flow ion-exchanged chromatography.By studying the antibacterial activities of the four group,excepting the precursor protein,the other groups had a good inhibition on the salmonella,staphylococcus aureus and escherichia coli,especially the group II and III.

关 键 词：鲢鱼 超声波辅助提取 鱼精蛋白 离子交换纯化 抑菌 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]