高产乙醇酵母菌株的筛选及产醇发酵条件研究  

Screening and optimization of a high ethanol-producing engineering Saccharomyces strain

作　　者：杜仁鹏 赵丹[1,3] 宋刚 王瑶[1] 张玉环 平文祥[1,3] 葛菁萍[1,3] 

机构地区：[1]黑龙江大学生命科学学院微生物省高校重点实验室,哈尔滨150080 [2]天津大学化工学院,天津300072 [3]黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨150500

出　　处：《黑龙江大学自然科学学报》2017年第5期575-583,共9页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(31590492;31270534;31470537);黑龙江省高等学校科技创新团队项目(2012td009);哈尔滨市科技局青年后备人才项目(2014RFQXJ101)

摘　　要：酿酒酵母(Saccharomyces cerevisiae)具有乙醇耐受力高、发酵工艺成熟、生物安全性高等优点,广泛应用于生物乙醇的生产。但其不能有效地利用木糖,不满足工业化大规模生产的要求。本研究以实验室现有重组菌株为研究对象,从中筛选出能够有效利用木糖的乙醇高产菌株ZLYRHZ7,并对其产醇发酵条件进行了研究。结果表明,当培养基中葡萄糖和木糖含量比为1∶4,酵母浸粉5 g·L^(-1)、磷酸氢二钾2.5 g·L^(-1)、硫酸镁0.25 g·L^(-1)、硫酸铵2.5 g·L^(-1)、氯化钙0.25 g·L^(-1),pH 5.0,采用针孔胶塞,200 r·min^(-1)发酵72 h时,乙醇产量达到最高,为25.48±0.23 g·L^(-1)。该研究结果为生物乙醇的工业化生产提供菌株来源,有助于酵母菌株木糖代谢流分析。With advantages of high ethanol tolerance, mature fermentation process and high biosecurity, Saccharomyces cerevisiae has been widely used to produce ethanol. However, Saccharorayces cerevisiae cannot effectively utilize xylose to meet the industrial requirements. In this study, a high ethanol-produ- cing engineering strain ZLYRHZ7, which could utilize xylose, was screened out from existing engineering strains in our laboratory. The optimal fermentation condition was as follows： pinhole rubber plug was used for fermentation, glucose/xylose was 1 ： 4, yeast extract powder was 5 g · L^-1, K2 HP04 was 2.5 g ·L^- 1,MgSO4 was 0.25 g· L^-1, （ NH4 ） 2 SQ was 2. al speed of the shaker was 200 r·min^-1 at fe had the highest ethanol production, up to 25. for industrial production of bioethanol, and strains. 5 g·L^-l, CaC12 rmentation 72 h. 48 ± O. 23 g·L^-1 was 0.25 g·L^-1, pH was 5.0, rotation- ZLYRHZ7, obtained by protoplast fusion, 1 The results provide a source of strains contribute to the analysis of xylose metabolism in yeast strains.

关 键 词：酵母 筛选 木糖 乙醇 优化 

分 类 号：TS261[轻工技术与工程—发酵工程]