葡萄糖对热带假丝酵母菌株SFX-Y9木糖醇发酵的影响  

Effect of glucose on xylitol fermentation by Candida tropicalis SFX-Y9

作　　者：赵祥颖[1] 张立鹤[1] 韩延雷 杨丽萍[1] 田延军[1] 刘建军[1] ZHAO Xiang-ying;ZHANG Li-he;HAN Yan-lei;YANG Li-ping;TIAN Yan-jun;LIU Jian-jun(Shandong Provincial Key Laboratory of Food and Fermentation Engineering, Shandong Food Fermentation Industry Research ＆ Design Institute, Jinan 250013 ,China)

机构地区：[1]山东省食品发酵工业研究设计院山东省食品发酵工程重点实验室,山东济南250013

出　　处：《食品与发酵工业》2017年第11期107-111,共5页Food and Fermentation Industries

基　　金：山东省自主创新重大专项(2014XGC07001)

摘　　要：以热带假丝酵母SFX-Y9为研究对象,考察葡萄糖添加对木糖醇发酵的影响。研究发现,菌株SFX-Y9以葡萄糖为碳源培养种子,不仅可以提高菌体浓度、缩短培养时间,而且对木糖的转化基本没有影响;发酵培养基中添加少量葡萄糖可以提高木糖消耗速率、缩短发酵周期。采用5 L发酵罐通过精准控制过程溶氧和低流速流加葡萄糖可以进一步提高木糖消耗速率,同时可以提高木糖转化率约15%。在最佳供氧和葡萄糖为辅助碳源的条件下,菌株SFX-Y9木糖醇最大生产速率可达5.0 g/(L·h)以上,转化率可达80%以上,生产成本可以与化学加氢竞争,具有非常好的工业开发应用前景。The effects of glucose on xylitol fermentation by Candida tropicalis SFX-Y9 was studied in this paper.Results showed that strains SFX-Y9 with glucose as carbon source to cultivate seeds could shorten training time and improve the concentration of cells,and had little influence upon conversion of xylose.When the fermentation medium was supplemented with a small amount of glucose,it could increase the consumption rate of xylose and shorten the fermentation cycle.Through the control of dissolved oxygen and the glucose fed-batch could further increase the rate of xylose consumption in 5 L fermentor,and the conversion rate of xylose increased by about 15%.Under the condition of optimal oxygen supply and glucose as a secondary carbon source,the production rate of xylitol can reach 5.0 g/（ L·h）,and the rate of conversion can reach more than 80%.The production cost of xylitol by microbial conversion can compete with the chemical hydrogenation,and it has a bright application future.

关 键 词：热带假丝酵母 木糖醇 葡萄糖 耗糖速率 转化率 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]