γ-聚谷氨酸生产菌种的ARTP诱变及其发酵条件优化  

The Production of γ-Polyglutamic Acid Strain by the ARTP and Optimization of it's Fermentation Conditions

作　　者：杜丹清 王刚[2] 庹有朋 万玉军[2] 李南臻 岳晓敏[2] 罗丽娟 DU Dan-qing;WANG Gang;TUO You-peng;WAN Yu-jun;LI Nan-zhen;YUE Xiao-min;LUO Li-juan(Sichuan Engineering Consulting Research Institute, Chengdu Sichuan 610000, China;Sichuan Academy of Food and Fermentation Industries ,Chengdu Sichuan 613330,China)

机构地区：[1]四川省工程咨询研究院,四川成都610000 [2]四川省食品发酵工业研究设计院,四川成都613330

出　　处：《食品与发酵科技》2017年第6期5-10,共6页Food and Fermentation Science & Technology

摘　　要：γ-聚谷氨酸是一种由生物所合成的氨基酸聚合物,具有极强的水溶性,生物相容性,能被广泛的运用到食品、医药、日化、环保、农业等行诸多领域,具有巨大的市场前景。本实验以实验室保藏的一株产γ-聚谷氨酸(γ-PGA)地衣芽孢杆菌Bacillus licheniformis QY-27为出发菌株,通过常压室温等离子体(ARTP)诱变,筛选获得一株遗传性能稳定的高产γ-聚谷氨酸突变菌株Bacillus licheniformis QS-21,该突变菌株γ-聚谷氨酸的产量较出发菌株的9.12g/L,提高了42.26%,达到15.89g/L。对筛选获得的变异株发酵条件优化结果显示,L-谷氨酸20g/L,甘油100g/L,Mg SO4·7H2O0.5g/L为变异株合适发酵培养基,在37℃,摇瓶转数230r/min,发酵培养72h,γ-聚谷氨酸的产量达22.56g/L,比优化前提高了29.56%。As a high molecular amino acid from biosynthesis, γ-PGA has high water solubility and biocompatibility, which can be widely used in food, medicine, household chemicals, environmental protection, agriculture and other fields, and thus has great market prospect. Bacillus licheniformis QY-27 preserved in the laboratory was used as original strain capable of producing γ-PGA, which was treated ARTP in order to obtain the high-yielding strain of γ-PGA, After the mutagenesis, one mutant named Bacillus licheniformis QS-21 formed, The yield of γ- PGA increased by 42.26%from 9.12g/L to 15.89g/L and showed high hereditary stability, through Optimized fermentation conditions, the results showed that the optimal fermentation medium was 1-glutamic acid 20g/L, glycerol 100g/L,MgSO4-7H2O 0.Sg/L, and the optimal fermentation condition was 37℃, 230r/min, cultivated for 72 hours Compared with before the optimization, the yield of γ-PGA increased by 29.56% to 22.56g/L.

关 键 词：地衣芽孢杆菌 Γ-聚谷氨酸 常压室温等离子体 诱变 发酵 优化 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]