油橄榄果渣水溶性膳食纤维的组成成分和功能特性分析  

Compositions and Functional Properties of Soluble Dietary Fiber from Olive Pomace

作　　者：丁莎莎[1] 黄立新[1] 张彩虹[1,2] 谢普军[1] 邓叶俊 王晓杰 

机构地区：[1]中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室,江苏南京210042 [2]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《林产化学与工业》2017年第6期110-116,共7页Chemistry and Industry of Forest Products

基　　金：江苏省自然科学基金资助项目(BK20151067)

摘　　要：以碱法制备的油橄榄果渣水溶性膳食纤维(SDF)粗品为研究对象,通过化学分析、气相色谱和红外光谱等方法分析其化学成分、单糖组成和官能团组成,并测定其体外吸附NO-2及抗氧化活性等功能特性。结果表明:油橄榄果渣SDF粗品中含SDF为60.90%,其单糖主要由木糖、阿拉伯糖、鼠李糖、葡萄糖、半乳糖和甘露糖组成,其比例为:64.0∶9.4∶3.9∶3.9∶2.6∶1.6,主要为木糖,其次为阿拉伯糖。红外光谱分析显示油橄榄果渣SDF粗品具有糖类的特征吸收峰;在体外模拟胃环境(p H值2)下,油橄榄果渣SDF对NO-2的最大吸附率达到98.92%,优于模拟肠环境(p H值7)下的8.69%;当SDF粗品质量浓度为6 g/L时,其对·OH的清除率达到最大为46.34%,弱于Vc;SDF粗品对DPPH·具有较强清除作用,其50%清除率对应的SDF粗品质量浓度(IC50)为0.06 g/L,其清除能力在低浓度时弱于Vc,在高浓度时接近Vc。The crude soluble dietary fiber（SDF） from Olive pomace （Olea europaea L. ） was obtained by using alkaline extraction method. The chemical composition, monosaccharide composition and functional groups were characterized by gas chromatography （GC） and infrared spectrometry. Furthermore, the effects of olive pomace SDF on the in vitro NOZ adsorption and antioxidant activity were investigated. The results showed that the content of SDF in the crude olive pomace SDF was 60. 90%. The monosaccharide of crude SDF was composed of xylose, arabinose, rhamnose, glucose, galactose and mannose with the molar ratio of 64. 0： 9.4： 3.9： 3.9： 2. 6： 1.6. The major component was xylose, followed by arabinose. Infrared spectra showed that the crude SDF had the characteristics absorption peaks of polysaccharide. The maximal adsorption capacity of SDF for NO2- in simulated gastric environment at pH =2（98.92% ） was greater than that in the intestine environment at pH =7（8.69% ）. The maximum scavenging ratio of the crude SDF for hydroxyl radical（ · OH） was 46. 34% at the mass concentration of 6 g/L and weaker thanthat of Vc. The crude SDF had strong DPPH radical scavenging activity with the IC50 of 0. 06 g/L. The DPPH radical scavenging activity of the crude SDF was weaker than that of Vc at low concentrations and close to that of Vc at high concentrations.

关 键 词：油橄榄果渣 水溶性膳食纤维 单糖组成 功能特性 

分 类 号：TQ35[化学工程] TS201.1[轻工技术与工程—食品科学]