响应面法优化微波辅助米渣蛋白糖基化改性工艺  

Response surface methodology for optimization of microwave-assisted technology parameters for glycosylation modification technology of rice dreg protein

作　　者：孟祥勇 张慧恩[2] 宋腾 张燕萍[3] 

机构地区：[1]安徽师范大学环境科学与工程学院,安徽芜湖241002 [2]浙江省生物工程重中之重学科,浙江万里学院,浙江宁波315100 [3]食品科学与技术国家重点实验室,江南大学,江苏无锡214122

出　　处：《食品工业科技》2018年第1期156-161,166,共7页Science and Technology of Food Industry

基　　金：国家自然科学基金青年科学基金(31701663);食品科学与技术国家重点实验室基金(SKLF-KF-201703);浙江省生物工程重中之重学科开放基金(KF2017001).

摘　　要：为了改善米渣蛋白的溶解性,提高蛋白质的利用率,本文以米渣蛋白和海藻酸钠为原料,利用微波对米渣蛋白进行接枝改性处理,比较分析海藻酸钠:米渣分离蛋白质量比、微波温度、微波时间、微波功率、p H对接枝度和褐变度的影响,并采用Central Composite中心组合实验设计,以接枝度为响应值,建立米渣分离蛋白与海藻酸钠接枝反应的二次回归模型,通过响应面分析和方差分析得出影响RDP与海藻酸钠接枝度的最优参数为:海藻酸钠:米渣分离蛋白质量比为1.88∶1(w/w)、p H10.18、微波功率186 W、微波温度77.7℃,在此条件下接枝度为36.87%,与实验设计中心点的最大值(37.03%)误差在5%范围内。本文所建立的米渣蛋白改性方法不仅用时短、效率高,而且改性效果好,具有较大的推广应用前景。In order to improve the solubility of rice dreg protein （RDP） and with the bioavailability, rice dreg protein （RDP） and sodium alginate were used as raw materials to synthesize RDP-sodium alginate graft with microwave heating.The ratio of sodium alginate to RDP,microwave temperature, time, power and pH on the modification effect were analyzed. On the basis of single factor experiments, response surface methodology combined with central composite design and variance analysis were employed to optimize process parameters.The optimum conditions of the grafting reaction were as follows：the ratio of sodium alginate to RDP 1.88：1 （w/w） ,pH10.18 ,microwave power 186 W, microwave temperature 77.7 ~C.Based on this condition,the theoretical degree of grafting was 37.03 %, which agreed well with the experimental value was 36.87%, and the error was within 5 % of the maximum value（37.03% ）.It turns out that the glycosylation modification technology of rice dreg protein which is gotten by microwave-assisted technology,is not only simple operation, economical and efficient, but also it has an interesting modification effect.The method is worth to extend and use vigorously in the modification of protein.

关 键 词：米渣蛋白 糖基化 改性工艺 响应面 

分 类 号：TS209[轻工技术与工程—食品科学]