荞麦粉对高脂血症小鼠血脂和肝脏抗氧化功能的调节作用  

Regulation Function of Buckwheat on Blood Lipid and Liver Anti-Oxidation of Hyperlipemia Mice

作　　者：綦文涛[1] 王世霞[1,2] 李笑蕊[1,2] 贠婷婷[1] 苗海江 李爱科[1] 

机构地区：[1]国家粮食局科学研究院,北京100037 [2]天津科技大学食品学院,天津300222

出　　处：《中国食品学报》2018年第2期63-70,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：粮食公益性行业科研专项(201313006-5)

摘　　要：目的:研究荞麦对高脂血症小鼠血脂和肝脏抗氧化的调节作用。方法:将雄性C57小鼠按体重随机分为基础对照组、高脂血症模型组和高脂血症条件下的10种荞麦处理组,每组10只。于实验第12周眼球采血,收集肝脏。测定血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDLC)含量;检测肝脏丙二醛(MDA)含量及总超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPH-Px)的活力变化,并观察小鼠肝组织病理变化。结果:与高脂血症模型组比,添加荞麦的高脂血小鼠,体重增长率和肝脏指数均呈降低趋势;荞麦降低了高脂血小鼠血液的TC、TG、LDL-C浓度,而HDL-C浓度升高;荞麦改善了高脂血症小鼠肝脏的GSH-Px和SOD活性,并显著降低MDA浓度(P﹤0.05);与高脂血症模型组相比,荞麦处理小鼠的肝组织细胞排列整齐,轮廓及索状结构清晰,细胞内脂滴空泡相对少。苦荞麦调节血脂和抗氧化作用优于甜荞麦。结论:荞麦具有降低高脂膳食引起的血脂升高作用;提高肝脏的抗氧化能力;改善肝脏脂代谢,起到保护肝脏的作用。Objective： To study the regulation of buckwheat on blood lipid and liver antioxidant in hyperlipemia mice. Method： Male C57 mice were divided into normal diet, hyperlipemia, and treatments groups. The treatments were carried out by 10 kinds of buckwheat under the condition of high-fat diet, with 10 mice in each group. Blood and liver samples were collected after 12 weeks. The levels of total cholesterol（TC）, triglycerides（TG）, high density lipoprotein cholesterol（HDL-C）, and low density lipoprotein cholesterol（LDL-C） in the serum were measured. The content of malondialdehyde（MDA） as well as the activities of total superoxide dismutase（SOD） and glutathione peroxidase（GPH-Px） were determined, and the pathological sections of the liver tissues were observed. Results： It was found that mice in all the buckwheat treated groups had lower body weights and decreased liver indexes when compared with mice in pure hyperlipemia group. The concentrations of TC, TG, and LDL-C in the serum were decreased, and the concentration of HDL-C was increased. Buckwheat improved GSH-Px and SOD activities of liver, and reduce the MDA concentration significantly（P〈0.05） in hyperlipemia mice. Meanwhile, the cellular arrangement, the outline and funicular structures of liver in hyperlipemia mice were improved by the addition of buckwheat. Moreover, the results showed that the improvement of blood lipid and antioxidant activity by tartary buckwheat was better than that by common buckwheat. Conclusion ：Buckwheat has the positive effects in reducing blood lipids, enhancing liver antioxidant capacities and improving lipid metabolism of liver, thus has potential functions in the protection of liver.

关 键 词：荞麦粉 高脂血症 血脂调节 抗氧化作用 

分 类 号：R151[医药卫生—营养与食品卫生学]