基因工程原理教学探索——以克隆策略教学为例  

Exploration into teaching and learning of principles of Genetic Engineering——Taking cloning strategies as an example

作　　者：刘增然[1] 张光一[1] 张香美[1] 赵士豪[1] 

机构地区：[1]河北经贸大学生物科学与工程学院,河北石家庄050061

出　　处：《微生物学通报》2018年第3期509-515,共7页Microbiology China

基　　金：河北经贸大学2015年度精品资源共享课建设项目;2017年度教学研究项目(2017JYY48);河北省科技计划项目(15225503D);河北经贸大学科研基金项目(2014KYZ05)~~

摘　　要：克隆策略是分子生物学研究和生物制品开发必不可少的工具,生物技术及相关专业的学生应该有扎实的理论基础和足够的应用经验。由于实践机会少,大学生做到学以致用很难。为了让学生更好地掌握克隆技术,我们在基因工程原理课程教学中采用了基本理论+论文案例的课堂教学模式进行克隆策略的教学。通过创设问题任务、拓展知识点,完成克隆策略基本原理和主要方法的课堂教学,使学生全面系统掌握基因克隆的流程和可用方法;通过教师引导的自学、讨论、自教完成应用论文的学习,使学生获得基于克隆策略设计实验的技能和方法选择的技能。教学实践证明,基本理论与论文案例嵌合的教学模式可以弥补课堂学习与实际应用之间的差距,使学生的实验设计技能、解决问题的能力得以提高,逻辑思维的严谨性、缜密性得以培养。Cloning strategies is the most essential tools for molecular biological studies and bio-product development and thus students in biological techniques and related majors should have a solid theoretical foundation and enough practical experience with this technique. As a fact, due to having less training and practice opportunity during their studies, it is difficult for students to apply what they have learned. Aiming to make the undergraduates better master the gene cloning strategies, we have adopted a model that combines theory with application case for the teaching-learning of cloning strategies in Genetic Engineering course. The cloning strategies were presented section by section with traditional classroom teaching and the undergraduates were made to fully grasp the process of gene cloning and available methods via designing questions and tasks and enlarging basic knowledge points. Additionally, research papers were introduced into classroom teaching-learning and the students got to know how to use cloning strategies to make an experimental design and select the methods that can be used via self-learning, discussion and self-presentation under instructor＇s guidance. Our practices show that the model combining cloning theory and research paper can bridge the gap between classroom learning and application, raise the students＇ experimental design and problem solving skills, and cultivate their logical thinking.

关 键 词：克隆策略 实验设计 应用能力 自主学习 

分 类 号：G642[文化科学—高等教育学] Q78-4[文化科学—教育学]