一株高产木聚糖酶菌株的筛选鉴定及酶学特性研究  

Screening,indetification and enzymatic properties of the high xylanase producing stain

作　　者：郑虹[1] 李莉娟 童秋霞 陈联邦 王艳君[1] 季潇炜 邓加聪[1,2] ZHENG Hong1 ,LI Li-juan1, TONG Qiu-xia1, CHEN Lian-bang WANG Yan-jun1 , JI Xiao- wei1 , DENG Jia-cong1 ,2,,(1.School of Ocean and Biochemical Engineering, Fuqing Branch of Fujian Normal University, Fuqing 350300, China; 2.Food Sofe Plastic Packaging Technology Fujian University Engineering Research Center,Fuqing 350300,Chin)

机构地区：[1]福建师范大学福清分校,海洋与生化学院,福建福清350300 [2]食品软塑包装技术福建省高校工程研究中心,福建福清350300

出　　处：《食品工业科技》2018年第6期126-129,共4页Science and Technology of Food Industry

基　　金：福建店自然科学基金面上项目(2015J01132;福建省教育厅中青年教师项目(JA15569);福建省国家自然基金应急管理项目(21547005).

摘　　要：以木聚糖为唯一碳源,在富集培养基的基础上,通过初筛和摇瓶复筛,筛选到一株高产木聚糖酶的菌株RX-9,对菌株RX-9进行菌落形态、生理生化实验及16S rRNA 序列遗传分析,该菌株的序列与Enterbacter(肠杆菌属)的多株菌具有99%以上的同源性,将该菌株归属为肠杆菌属(Enterbacter),命名为Enterbacter RX-9。对菌株所产木聚糖酶的酶学特性进行研究,发现该菌株所产的木聚糖酶在100℃下保温30 min,其酶活力仍达到2310.03 U/mL;当pH在7.5~9.5之间,木聚糖酶的活性基本稳定在3200 U/mL以上,表明该酶具有较好的热稳定性和pH稳定性。The strain RX-9 producing high xylanase was screened through preliminary screening and shake flask, based on enriched medium with xylan as the sole carbon source.The strain RX-9 had more than 99% homology to EnteroSacter,and was ascribed to Enterobacter sp.named as Enterbacter RX-9 by the colony morphology, physiological and biochemical analysis of experiment and genetic analysis of 16s rRNA sequence.The enzymatic properties of xylauase produced by the strain RX-9 was studied,and found that the enzyme activity was still 2310.03 U/mL kept at 100 ℃ for 30 rain, and The xylanase activity was above 3200 U/mL when pH was 7.5-9.5.The results showed that the xylanase had good thermal stability and pH stability.

关 键 词：木聚糖酶 筛选 鉴定 酶学特性 

分 类 号：TS201.3[轻工技术与工程—食品科学]