4种功能红曲相关标准中洛伐他汀含量测定方法的比较  

Comparison of lovastatin determination methods of four related standards of functional red koji

作　　者：李志强[1] 刘颖[1] 林风[1] 吴丽云[1] LI Zhiqiang;LIU Ying;LIN Feng;WU Liyun(Fujian Provincial Key Laboratory of Screening for Novel Microbial Products,Fujian Institute of Microbiology,Fuzhou 350007, Chin)

机构地区：[1]福建省微生物研究所福建省新药(微生物)筛选重点实验室,福建福州350007

出　　处：《中国酿造》2018年第5期184-188,共5页China Brewing

基　　金：福建省财政厅省直教育科研专项(闽财指[2015]1297号);福建省红曲微生物技术开发应用工程研究中心(闽发改高技【2017】513号);福州市仓山区科技创新公共服务平台项目(2015K03);福建省公益类科研院所专项(2015R1009-4;2017R1009-5;2018R1009-3;2018R1009-5)

摘　　要：功能红曲中的洛伐他汀是具有降脂功能的生物活性物质,其含量是功能红曲重要的质量指标之一。现阶段我国功能红曲产品相关标准中规定的洛伐他汀含量测定方法并不完全相同。为准确测定功能红曲中的洛伐他汀含量,该研究选取5份红曲,分别按4种功能红曲产品标准中规定的方法进行洛伐他汀含量测定,发现《中国药典》2015年版的方法会导致酸式洛伐他汀的保留时间异常、峰形不对称。其他3种标准中规定的高效液相色谱方法均可测定功能红曲中洛伐他汀酸式和内酯式构型的含量,但洛伐他汀总含量测定结果有较大差异,使用《四川省中药饮片炮制规范》2015年版的方法测定结果较好。Lovastatin, produced by functional red koji, is a beneficial bioactive substance with lipid-lowering function. The content of lovastatin is one of important quality indexes of functional red koji. However, the methods described in functional red koji related standards in China are differ-ent. In order to determine the content of lovastatin accurately, five functional red koji samples were selected, and the contents of lovastatin were determined according to four related standards. Results showed that using the method in China Pharmacopoeia the 2015 edition, the retention time of the acid forms of lovastatin was abnormal, and the acid forms of lovastatin showed the asymmetric peak shapes. Both acid and lactone forms of lovastatin were determined synchronously by HPLC methods in the other three standards, but there was much difference among the total contents of lovastatin determined by these methods. The method of Sichuan Standards for the Processing of Chinese Herbal Pieces the 2015 edition was a better one.

关 键 词：功能红曲 洛伐他汀 高效液相色谱 

分 类 号：TS202.3[轻工技术与工程—食品科学]