非酶促糖基化SOD对四氧嘧啶诱导糖尿病大鼠的降血糖作用  

Effect of Glycated SOD on Hypoglycemic Action in Alloxan-induced Diabetic Rats

作　　者：赵丹[1] 蔡丽萍[1] 傅婷[1] 张海玲[1] 刘树滔[1,2] 饶平凡 Zhao Dan;Cai Liping;Fu Ting;Zhang Hailing;Liu Shutao;Rao Pingfan(Institute of Biotechnology,Fuzhou University,Fuzhou 350108;Shanghai Institutes for Biological Sciences of Chinese Academy of Scienees-Zhejiang Gong Shang University Joint Centre for Food and Nutrition Research,Hangzhou 310035;Key Laboratory of Opto Electronic Science and Technology for Medicine of Ministry of Education,Fujian Provincial Key Laboratory of Photonics Technology,Fujian Normal University,Fuzhou 350007)

机构地区：[1]福州大学生物工程研究所,福州350108 [2]医学光电科学与技术教育部重点实验室福建省光子技术重点实验室福建师范大学,福州350007 [3]中国科学院上海生命科学研究院-浙江工商大学食品营养科学联合研究中心,杭州310035

出　　处：《中国食品学报》2018年第6期19-26,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31071497;31271859);医学光电科学与技术教育部重点实验室(福建师范大学)福建省光子技术重点实验室开放课题(JYG1704)

摘　　要：为了探究美拉德反应对超氧化物歧化酶(SOD)的辅助降血糖的影响,以牛血SOD为原料,加入葡萄糖(Glu)通过美拉德反应进行非酶促糖基化。将SOD糖基化产物(SOD-G)与单独牛血SOD分别作用于由四氧嘧啶诱导的糖尿病大鼠,对比观察一定剂量的SOD与等剂量的SOD-G,以及不同剂量的SOD-G对糖尿病大鼠体重、血糖,口服糖耐量(OGTT)、组织(心、肝、肾、胰腺、骨骼肌)SOD水平、血浆胰岛素(INS)、腺苷酸活化蛋白激酶(AMPK)、丙二醛(MDA)水平的影响。试验结果表明:SOD-G和SOD对四氧嘧啶诱导的I型糖尿病大鼠有显著的辅助降血糖效果,且各有优势。在大鼠体重控制上两者无差异。SOD-G的降血糖效果明显好于SOD(P<0.05),而在OGTT试验中,SOD对糖耐量损伤修复效果明显好于SOD-G(P<0.05)。组织SOD水平试验中,两者均提升了胰腺中的SOD含量,对骨骼肌和心脏中SOD含量无显著影响。SOD-G组的肝脏和肾脏SOD水平显著低于SOD组,两者在INS、AMPK、MDA水平上无显著差异。这些结果说明SOD在发生美拉德反应修饰后,仍具有明显辅助降血糖功效,且总体效果SOD-G好于SOD。这可能是由于美拉德反应修饰后其结构发生变化,SOD-G与SOD在体内的吸收作用方式随之变化,导致最终的治疗效果存在差异。In order to explore the impact of auxiliary hypoglycemic of superoxide dismutase（SOD） by Maillard reaction, based on bovine blood SOD as raw material, added glucose for non-enzymatic glycation reaction, the glycated SOD（SOD-G） and single bovine blood SOD were used to treat alloxan-induced diabetic rats, to compare the effect of weight, blood glucose, oral glucose tolerance, the SOD levels of tissue organs（Heart, liver, kidney, pancreas and skeletal muscle） and the level of plasma insulin（INS）, amp activated protein kinase（AMPK） and malondialdehyde（MDA）of diabetic rats given SOD and an equal dose of SOD-G, as well as different doses of SOD-G. The test results show that SOD-G and SOD have a significant effect on reduction of blood glucose in alloxan-induced type I diabetic rats,but each has its advantages. There are no differences in body weight control. SOD-G hypoglycemic effect is significantly better than SOD（P〈0.05）, while in the OGTT test, the effect of SOD on glucose tolerance damage is significantly better than SOD-G（P〈0.05）. In tissue SOD level test, both of them increase the content of SOD in the pancreas, and have no significant effect on the SOD content in skeletal muscle and heart, and SOD level of liver and kidney in SOD-G group is significantly lower than that in SOD group, There are no significant difference in INS, AMPK, and MDA levels.These results indicate that superoxide dismutase, in the wake of the Maillard reaction modification, still has obvious auxiliary hypoglycemic effect, and the overall effect of SOD-G is better than SOD. But maybe due to the Maillard reaction modification its structure changes, and SOD-G and SOD in vivo absorption way changes, there are some differences in the final treatment effect.

关 键 词：SOD 美拉德反应 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]