嗜热乳酸菌与酸性蛋白酶协同提取玉米淀粉  

Lactobacillus thermophilus and Acid Protease Synergistic Extraction Corn Starch

作　　者：马恒[1] 刘庆艾 马耀宏 杨俊慧 杨伟超[2] 史建国 MA Heng;LIU Qing＇ai;MA Yaohong;YANG Junhui;YANG Weichao;SHI Jianguo(Shandong Provincial Key Laboratory for Biosenseors,Biology Institute,Qilu University of Technology（Shandong Academy of Sciences）（Jinan 250014;Institute of Applied Ecology,Chinese Academy of Sciences（Shenyang 110000)

机构地区：[1]齐鲁工业大学(山东省科学院),山东省科学院生物研究所,山东省生物传感器重点实验室,济南250014 [2]中国科学院沈阳应用生态研究所,沈阳110000

出　　处：《食品工业》2018年第7期62-66,共5页The Food Industry

基　　金：山东省科学院青年科学基金(2015QN009),山东省科学院科技发展基金项目(科基合字2017第37号); 山东省自然科学基金中青年科学家科研奖励基金(ZR2016CB42); “863”计划子课题(2015AA021005)

摘　　要：在玉米浸泡的过程中,采用嗜热乳酸菌与酸性蛋白酶协同浸泡的方法提取玉米淀粉,并优化该工艺。通过单因素试验研究初始嗜热乳酸菌菌液浓度、嗜热乳酸菌浸泡时间、加酶量和酶作用时间对玉米淀粉得率的影响,通过正交试验优化组合得到最佳工艺条件:初始嗜热乳酸菌浓度16%、嗜热乳酸菌浸泡时间26 h、加酶量1 100 U/g、酶作用时间24 h。在最优条件下,淀粉得率由传统工艺的56.02%提高到64.26%。此浸泡工艺取缔了亚硫酸的使用,在较低酶添量的情况下,利用嗜热乳酸菌与酸性蛋白酶协同作用,能有效减少能源消耗及环境污染、提高玉米淀粉及其副产物的利用率。During the process of corn steeping, adopt collaborative soaking of Lactobacillus thermophilus and acid protease to synergistic extraction corn starch, and optimize the technology. The effects of the initial concentration and soaking time of Lactobacillus thermophilus, as well as the content and action time of acid protease on the yield of corn starch were studied by single factor experiments. The optimum technological condition was identified by orthogonal test as the initial concentration of lactic acid for 16%, the soaking time of Lactobacillus thermophilus for 26 h, the content of acid protease for 1 100 U/g, and the action time of acid protease for 24 h. Under the optimum condition, the yield of starch was increased from 56.02% to 64.26%. The soaking process outlawing the use of sulfite, in the case of lower enzyme dosage, protease and lactic acid produced byLactobacillus thermophiles could effectively reduce energy consumption and environmental pollution, and improve the utilization ratio of corn starch and its by-products.

关 键 词：嗜热乳酸菌 酸性蛋白酶 玉米浸泡 淀粉得率 

分 类 号：TS235.1[轻工技术与工程—粮食、油脂及植物蛋白工程]