响应面法优化“金花”菌培养基配方  

Optimization of the Formulation of Liquid Media for the Culture of Aspergillus Cristatus by Response Surface Method

作　　者：周国庆 谭玉梅 王亚萍[2] 黄永会 任锡毅[3,2] 刘永翔 刘作易[4] ZHOU Guo-qing;TAN Yu-mei;WANG Ya-ping;HUANG Yong-hui;REN Xi-yi;LIU Yong-xiang;LIUZuo-yi(College of Life Sciences,Guizhou University,Guiyang,Guizhou 550025,China;Institute of Biotech-nology,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China;Key Laboratory of Agricultural Biotechnology of Guizhou Province,Guiyang,Guizhou 550006,China;Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006,China)

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省农业生物技术重点实验室,贵州贵阳550006 [3]贵州省农业科学院生物技术研究所,贵州贵阳550009 [4]贵州省农业科学院,贵州贵阳550006

出　　处：《山地农业生物学报》2018年第2期27-32,共6页Journal of Mountain Agriculture and Biology

基　　金：贵州省科技支撑计划黔科合支撑([2017]2561);黔农科院院专项([2016]028号);贵州省农业科学院专项(GAAS-SP-2014/004)

摘　　要：采用单因素试验和响应面法对冠突曲霉液体培养基进行优化,以子囊孢子浓度为响应值。通过单因素实验确定最佳含水量、氯化钠浓度、接种量分别是10 ml、5%、240μl。使用Design expert 8中的Box-Behnken design(BBD)进行试验设计,采用响应面分析法优化出液体培养基最佳配方是在10 g麸皮的基础上,添加Na Cl 6.13%(0.613 g),9.79 m L无菌水,接种246.48μl浓度为108个/ml"金花"菌孢子悬液,28℃条件下,培养7 d,发酵物浸泡过滤处理得孢子含量达3.51×10~7个/m L。优化后仅用简单、经济的麸皮培养基就能快速培养得到高浓度的金花菌孢子,这些孢子按需求洗脱稀释后即可用于人工接种,这促进了金花菌人工接种发花的普及,同时为培养基的优化提供了参考。Response surface method and single-factor experiment were carried out to optimize the formulation of culture media of Aspergillus cristatus to obtain a maximum spore concentration. By three single-factor de-signs,the best amount of moisture content,NaCl concentration and inoculum amount are 10ml,5% and 240μl,respectively. According to the Box-Behnken design（BBD）in Design Expert,the optimal liquid media formulation was determined by response surface analysis as 10g of sifted bran consisting of 9.79 ml water,6.13% NaCl and 246.48 μl spore suspension which concentration was about 108 spores / ml. After 7 days of cul-ture at 28℃, the maximum spore concentration was approximately 3.51×107 spores/ml. This research could facilitate the popularization of artificial inoculation, providing reference for future study on the preparation and optimization of liquid media for the culture of “Golden flower” fungus.

关 键 词：茯砖茶 金花菌 冠突曲霉 响应面法 发酵剂 

分 类 号：Q939.9[生物学—微生物学]