PCR技术结合试纸条法快速检测乳粉中的克鲁诺杆菌  

PCR combined with the lateral flow assay for rapid detection of Cronobacter spp. in milk powdered

作　　者：姜霞[1] 王蕊[1] 潘瑞丽[1] 孙露宏 李明雨[1] 党芳芳 赵玥明 曲艳艳[1] 满朝新[1] 姜毓君[1,2] JIANG Xia;WANG Rui;PAN Ruili;SUN Luhong;LI Mingyu;DANG Fangfang;ZHAO Yueming;QU Yanyan;MAN Chaoxin;JIANG Yujun(Northeast Agricultural University a.Key Lab of Dairy Science,Ministry of Education,College of Food Scienc;b.National P.esearch Center of Dairy Engineering and Technology/Northeast Agricultural University,Harbin 150030,Chin)

机构地区：[1]东北农业大学食品学院乳品科学教育部重点实验室,哈尔滨150030 [2]东北农业大学国家乳业工程技术研究中心,哈尔滨150030

出　　处：《中国乳品工业》2018年第7期41-43,共3页China Dairy Industry

基　　金：黑龙江省杰出青年基金项目(JC201415);黑龙江省院所创新能力提升专项计划项目(YC13D005);东北农业大学学术骨干(15XG26)

摘　　要：建立了一种PCR结合试纸条的方式快速检测婴幼儿奶粉中的克罗诺杆菌的方法,利用克鲁诺杆菌的omp A基因进行PCR特异性检测,优化并建立了横向流动试纸条法特异性捕获PCR扩增产物,从而快速检测婴幼儿奶粉中的克鲁诺杆菌。结果表明,PCR技术结合试纸条方法针对7株菌进行特异性实验,特异性良好,在纯培养物中的检测限为5.6×10~1mL^(-1),人工污染婴幼儿配方奶粉中的克鲁诺杆菌的检测限为5.6×10~2g^(-1)。该方法具有特异性强,灵敏度高,操作简单,快捷,在致病菌的快速检测中具有潜在的研究价值。The system based on PCR combined with the lateral flow assay was established for rapid detection of Cronobacter spp. in powdered infant formula. In this investigation PCR method was optimized based on the omp A gene for sensitive and special rapid detection of Cronobacter spp., of which the lateral flow assay was applied to capture the PCR amlicons. The results revealed that the detection limit for detection of Cronobacter spp. in pure culture was 5.6×10^1mL^-1, simultaneously the detection limit for detecting Cronobacter spp. contaminated in powered infant fomula was 5.6×10^2g^-1. The probe-free label system was rapid, sensitive and easy-to-operate and potentially used for the rapid detection of the foodborne pathogens.

关 键 词：婴儿配方乳粉 PCR 试纸条 克鲁诺杆菌 快速检测 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]