鲫鱼皮胶原肽对小鼠的促钙吸收作用  

Effect of Collagen Peptides from Skin of Carassius auratus on Promoting Calcium Absorption in Mice

作　　者：刘艳双 侯焘[1,2] 郭丹郡 刘维维 石文[1,2] 何慧 LIU Yanshuang;HOU Tao;GUO Danjun;LIU Weiwei;SHI Wen;HE Hui(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;Key Laboratory of Environment Correlative Dietology,Ministry of Education,Wuhan 430070,China)

机构地区：[1]华中农业大学食品科学与技术学院,湖北武汉430070 [2]环境食品学教育部重点实验室,湖北武汉430070

出　　处：《食品科学》2018年第13期241-245,共5页Food Science

摘　　要：目的:研究胶原肽(collagen peptides,CPs)对小鼠的促钙吸收作用。方法:采用小鼠低钙膳食模型法,以昆明雄性小鼠为实验动物,设置正常组、低钙模型组、碳酸钙组、CPs低剂量(500 mg/kg mb+CaCO_3)、高剂量(1 000 mg/kg mb+CaCO_3)组、酪蛋白磷酸肽对照组,连续灌胃6周,考察小鼠血清钙、磷、碱性磷酸酶(alkaline phosphatase,ALP)活力、钙吸收率、各项骨指标及骨微观结构、骨小梁形态变化。结果:CPs低、高剂量组小鼠的ALP活力显著低于低钙模型组(P<0.05),其骨长度、干质量指数、骨钙含量、骨矿物密度(bone mineral density,BMD)、骨矿物含量(bone mineral content,BMC)均显著高于低钙模型组(P<0.05),CPs低剂量组可使喂食低钙饲料的小鼠的各项指标达到正常组小鼠的水平。CPs低剂量组小鼠的ALP活力显著低于碳酸钙组(P<0.05),其骨钙含量、BMD、BMC均显著高于碳酸钙组(P<0.05),提示补充碳酸钙的同时补充CPs,其促钙吸收效果更好。结论:CPs可提高小鼠钙吸收率,增加骨小梁数目及强度,促进骨骼生长,从而能有效促进钙的吸收。This study was designed to investigate the effect of collagen peptides（CPs） on calcium absorption in mice. The animals were randomly divided into normal control group, low calcium model group, calcium carbonate（CaCO3） group, low-（500 mg/kg mb ＋ Ca CO3） and high-dose（1 000 mg/kg mb ＋ CaCO3） CPs treatment groups and casein phosphopeptides（CPPs） control group. After 6 weeks of oral administration, the serum levels of calcium, phosphorus and alkaline phosphatase（ALP）, calcium absorption rate, bone indices, bone microstructure and trabecular bone morphological changes were measured. The results showed that ALP activity of the CPs treatment groups was significantly lower than that of the low calcium model group（P〈0.05）, while bone length, dry mass index, bone calcium content, bone mineral density（BMD）, and bone mineral content（BMC） were significantly higher（P〈0.05）. CPs at low dose could reverse almost all indexes of the low calcium model group to normal. We observed a significant decrease in ALP activity and a significant increase in bone calcium content, BMD and BMC for the low dose CPs group compared with the CaCO3 group（P〈0.05）, suggesting that supplementation of both calcium carbonate and CPs could promote calcium absorption and improve bone microstructure and trabecular morphology. Therefore, CPs could increase calcium absorption rate by increasing the number and intensity of trabecular bone and promoting bone growth.

关 键 词：鲫鱼皮 胶原肽 钙吸收 低钙膳食模型 骨微观结构 

分 类 号：TS201.2[轻工技术与工程—食品科学]