ε-聚赖氨酸高产改造菌发酵过程分析与工艺改进  

Analysis of fermentation process and technological improvement of ε-poly lysine high-yield mutant Streptomyces albulus GS114

作　　者：张龙[1] 郑根成 潘龙 陈旭升[1] 毛忠贵[1] ZHANG Long;ZHENG Gencheng;PAN Long;CHEN Xusheng;MAO Zhonggui(The Key Lab of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]工业生物技术教育部重点实验室,江南大学生物工程学院,江苏无锡214122

出　　处：《工业微生物》2018年第3期17-23,共7页Industrial Microbiology

基　　金：江苏省产学研前瞻性联合研究项目(BY2016022-25)

摘　　要：ε-聚赖氨酸(ε-PL)是我国新近批准的一种天然食品防腐剂,由链霉菌好氧发酵制备而来。通过传统育种手段强化产生菌ε-PL合成能力是提高其发酵水平的重要途径,然而高产改造菌与出发菌株发生的生理改变却很少被关注。本研究从培养特征,营养需求和发酵过程参数等方面进行比较与分析,发现高产菌株Streptomyces albulus GS114具有需氧量小、菌体量低、ε-PL产量高、单位菌体ε-PL合成能力强、转化率高等特点。为了进一步提高S.albulus GS114的ε-PL产量,通过提高pH冲击策略中预培养p H增加其菌体量,实现ε-PL产量达到53.49 g/L,较优化前提高了11.9%。研究结果将为ε-PL工业化生产奠定基础。ε-Poly-L-lysine（ ε-PL） is a kind of natural food preservative recently approved in China,and it is prepared by Streptomycetes through aerobic fermentation. It is an important way to improve the fermentation level of producing strain ε-PL by traditional breeding methods. However,less attention was paid to the physiological differences between the original strain and the high-yield mutant. In this study,cultivation characterization,nutrition requirement and fermentation process parameters between the original strain M-Z18 and the mutant Streptomyces albulus GS114 were compared. Results showed that the GS114 exhibited less oxygen consumption,lower cell weight,and higher ε-PL production,ratio of ε-PL production/DCW and ε-PL yield. In order to further improve the ε-PL production,an approach aimed at increasing DCW of GS114 in 5L fermenter by the increase of pre-culture pH value was proposed. As a result,ε-PL production reached 53. 49 g/L,which was improved by 11. 9% than the control. These results provided guidance for future ε-PL industrial production.

关 键 词：食品防腐剂 Ε-聚赖氨酸 链霉菌 补料分批发酵 pH冲击策略 

分 类 号：TQ920.6[轻工技术与工程—发酵工程] TS202.3