不同蛋白酶酶解河蚬产物的抗氧化活性研究  

Study on Antioxidant Activity of Freshwater Clam Hydrolysates Produced by Different Proteases

作　　者：朱小燕 方芳[1] 任世英 周佳 谭中标[1,2] 李相前 ZHU Xiao-yan;FANG Fang;REN Shi-ying;ZHOU Jia;TAN Zhong-biao;LI Xiang-qian(Faculty of Life Science and Food Engineering,Huaiyin Institute of Technology,Huaian Jiangsu 223003,China;Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration,Huaiyin Institute of Technology,Huai＇an Jiangsu 223003,China)

机构地区：[1]淮阴工学院生命科学与食品工程学院,江苏淮安223003 [2]淮阴工学院江苏省生物质转化与过程集成重点实验室,江苏淮安223003

出　　处：《淮阴工学院学报》2018年第3期30-35,共6页Journal of Huaiyin Institute of Technology

基　　金：淮安市科技支撑项目(HAN2015026);江苏省产学研前瞻性联合研究项目(BY2016061-05);国家自然科学基金项目(21706089);国家自然科学基金项目(21576110)

摘　　要：以河蚬为原料,采用Alcalase、Neutrase、Flavourzyme、Papain四种蛋白酶水解河蚬,考察不同酶酶解产物的抗氧化活性包括DPPH自由基清除能力、超氧阴离子自由基(O-2·)清除能力、羟自由基(·OH)清除能力及还原力。研究发现,Alcalase2 h酶解产物DPPH自由基清除能力最佳,清除率为83.17%,Alcalase8 h酶解产物O-2·的清除率最高达到72.52%。Papain6 h酶解产物·OH清除效果最佳,清除率达83.86%,Papain 6 h酶解产物还原力最高,酶解产物OD700nm值为0.74。可见蛋白酶种类和酶解时间对河蚬酶解产物的抗氧化活性具有较大影响,且抗氧化活性指标不一样,不同的蛋白酶酶解河蚬产物的抗氧化活性也有较大差异。Four kinds of protease,Alcalase,Neutrase,Flavourzyme and Papain,were used for the hydrolysis of freshwater clam,and antioxidant activities of hydrolysates in terms of 1,1-diphenyl-2-picrylhydrazyl（ DP PH） radical scavenging activity,superoxide radical anion（ O2^-·） scavenging activity,hydroxyl radical（ ·OH） scavenging activity and reducing power were evaluated. The results showed that hydrolysate produced by Alcalase at 2 h exhibited the best DPPH radical scavenging ability and its scavenging activity reached 83. 17%.Hydrolysate produced by Alcalase at 8 h showed the highest O2^-· scavenging activity and it was 72. 52%. Hydrolysate produced by Papain at 6 h exhibited the best · OH scavenging ability and its scavenging activity reached 83. 86%. Hydrolysate produced by Papain at 6 h displayed highest reducing power and its OD700 nm value was 0. 74. The results showed that the protease type and hydrolysis time had significant effects on the antioxidant activity of the hydrolysate. The antioxidant activity of hydrolysates produced by different protease were different from each other using the different indicators of antioxidant activity. Overall,these results could provide theoretical basis for preparation and use of freshwater clam hydrolysate with antioxidant activity.

关 键 词：河蚬 蛋白酶 酶解产物 抗氧化活性 

分 类 号：Q519[生物学—生物化学] TS254.4[轻工技术与工程—水产品加工及贮藏工程]