可得然胶生产菌种的筛选及发酵条件优化  

Isolation of a Curdlan-Producing Bacterium and Optimization of Fermentation Medium

作　　者：王萧玉竹 董晋军[1,2] 许国超[1,2] 韩瑞枝[1,2] 倪晔[1,2] WANG Xiaoyuzhu, DONG Jinjun, XU Guochao, HAN Ruizhi, NI Ye(1. Key Laboratory of Industrial Biotechnology. Ministry of Education, Jiangnan University, Wuxi 214122. China ; 2. School of Biotechnology, Jiangnan University, Wuxi 214122, Chin)

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《食品与生物技术学报》2018年第7期732-738,共7页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(21276112;21506073);江苏省自然科学基金项目(BK20150003)

摘　　要：采用苯胺蓝平板筛选法从土壤中筛选并鉴定了一株产可得然胶的根瘤菌,并对该菌株发酵合成可得然胶的培养基进行优化。在单因素优化的基础上,设计4因素3水平的正交优化方案,优化结果显示,该菌株发酵产可得然胶的最优培养基组成为:葡萄糖50 g/L,(NH4)2HPO42g/L,KH2PO41.5 g/L,Mg SO41.25 g/L,Fe SO4·7H2O 0.05 g/L,Mn SO40.02 g/L,Co Cl2·6H2O 0.01 g/L,Zn Cl20.01 g/L。经摇瓶发酵验证,可得然胶的产量和糖转化率较优化前分别提高了29.2%和5.4%,为进一步利用该根瘤菌发酵合成可得然胶奠定了良好基础。A Rhizobium sp. capable of producing curdlan was isolated from soil. The polysaccharide product was identified to be curdlan by testing the thermal property, molecule composition, infrared spectrum and molecule weight. Furthermore, the orthogonal optimization experiment was conducted to optimize the curdlan fermentation medium, and the best medium composition was 50 g/L,（NH4）2HPO42g/L,KH2PO4 1.5 g/L,MgSO4 1.25 g/L,FeSO4·7H2O 0.05 g/L,MnSO4 0.02 g/L,CoCl2·6H2O 0.01 g/L,ZnCl2 0.01 g/L Consequently, the curdlan production and glucose conversion rate were enhanced by 29.2% and 5.4% compared with those before optimization.

关 键 词：可得然胶 正交试验 根瘤菌属 苯胺蓝 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]