耐高温、高醇醋酸菌筛选、鉴定及低能离子选育  

Screening, Identification of a Thermoresistant and Alcohol-tolerant Acetic Acid Bacterium and Breeding of Imbecile Ion Beam

作　　者：李文[1] 王陶[1] 谢昊讯 高明侠[1] 董玉玮[1] 李同祥[1] 张传丽[1] Li Wen;Wang Tao;Xie Haoxun;Gao Mingxia;Dong Yuwei;Li Tongxiang;Zhang Chuanli(Xuzhou Institute of Technology,Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe,Xuzhou 221008,Jiangsu)

机构地区：[1]徐州工程学院江苏省食品资源开发与质量安全重点建设实验室,江苏徐州221111

出　　处：《中国食品学报》2018年第8期107-114,共8页Journal of Chinese Institute Of Food Science and Technology

基　　金：江苏省重点研发计划项目(BE2016648);江苏省高校自然科学研究重大项目(16KJA210001);江苏省"333"工程科研项目(BRA2016274)

摘　　要：从腐烂的猕猴桃中初筛醋酸菌,于不同浓度乙醇的液体培养基、不同温度下驯化,得到一株在高温、高醇条件下产酸量较高的菌株WT08。该菌株在含6%(体积分数,下同)乙醇的培养基中32℃培养72 h,产酸量达39.09 g/L;在含12%乙醇的培养基中32℃培养72 h,产酸量为12.99 g/L;在产酸基础培养基中,40℃培养72 h,产酸量达到5.89 g/L。对该菌株进行形态学观察、生理生化及分子生物学鉴定,初步确定该菌株为巴氏醋杆菌。利用能量为10 keV,剂量为70×2.6×10^(13)ions/cm^2的低能N^+注入WT08菌株,在后代中选育得到耐醇、耐高温性能都提高的菌株WT08-18,该菌株最高产酸达49.86 g/L,在含12%乙醇的培养基中产酸达15.16 g/L;在基础培养基中40℃培养72 h,产酸量达10.96 g/L,且该菌株有良好的遗传稳定性。将WT08-18菌株液体扩大培养,以体积分数3%的接种量接入成熟醋醅中,按照传统酿制工艺酿造,醋酸产量比传统酿制的提高30.54%,酒精转酸率提高18.81%,具有广泛的应用前景。In this study, Acetobocter was firstly screened from the decayed kiwifruit, which were cultured in liquid medium with different concentrations of ethanol at different temperatures, WT08 stain with the highest acetic acid pro- duction at the conditions of high temperature and high alcohol was screened, whose acetic acid production reached 39.09 g/L when cultured in the medium with 6% ethanol at 32 °C for 72 h, 12.99 g/L when cultured with 12% ethanol, and 5.89 g/L when cultured in the minimum medium at 40°C for 72 h. It was initially characterized as Acetobacter pasteuri- anus by testing its morphology, physiology, biochemistry and 16srDNA nucleotide sequence. WT08-18 was obtained from the strain WT08 by N＋（10 keV, 70×2.6×10＇3 ions/cm2） implantation and heredity of the strain was stable. The yield of total acid was 49.86 g/L, the yield of acid was 15.16 g/L under 12% ethanol and the yield of acid was 10.96 g/L quies- cently cultured 72 h at 40°C by WT08-18. The liquid state seed of WT08-18 was inoculated into the solid fermentation substrate of Hengshun Wantong aromatic vinegar. The results showed that the yield of acetic acid and the conversion rate of alcohol to acetic acid had increased by 30.54% and 18.81% respectively. It had potential application value in the in- dustrial production.

关 键 词：醋酸菌 低能离子 修饰 耐高温 耐高乙醇 

分 类 号：TS201.3[轻工技术与工程—食品科学] TS264.22[轻工技术与工程—食品科学与工程]