木醋杆菌发酵大豆糖蜜产细菌纤维素的研究  

Studies on Bacterial Cellulose Producted by Acetobacter xylinum Ferment Soybean Molasses

作　　者：程建军[1] 马佳歌 尹园[1] 徐速[1] 倪春蕾 张珊[1] Cheng Jianjun;Ma Jiage;Yin Yuan;Xu Su;Ni Chunlei;Zhang Shan(College of Food Science,Northeast Agricultural University,Harbin 150030)

机构地区：[1]东北农业大学食品学院,哈尔滨150030

出　　处：《中国食品学报》2018年第8期125-135,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家科技支撑计划课题(2014BAD22B01-04-03)

摘　　要：利用木醋杆菌1.1812发酵大豆糖蜜,生产细菌纤维素(BC)。采用单因素和响应面法优化发酵培养基,通过傅里叶红外光谱、扫描电子显微镜和拉伸强度测定仪对细菌纤维的结构和性质进行表征。结果表明,木醋杆菌发酵大豆糖蜜的优化培养基配方:糖蜜可溶性固形物含量4.45°Brix、氮源(m^(蛋白胨):m_(酵母浸粉)=1∶1)24.39 g/L、硫酸镁1.42 g/L、磷酸氢二钠1.95 g/L、柠檬酸0.98 g/L。在此条件下发酵5 d,BC产量为(5.85±0.27)g/L(干基),是基本培养基BC产量【(2.72±0.16)g/L】的2.15倍。与相同条件下基本培养基BC相比,大豆糖蜜培养基不改变BC的化学基团。随着发酵时间的延长,大豆糖蜜BC纤维网状结构愈加致密,拉伸强度显著提高,持水性基本一致(P<0.05)。In this study, bacterial cellulose （BC） was prepared by Acetobacter xylinum strain 1.1812 using soybean molasses as a carbon source. The single factor experiments and response surface methodology were used to optimize the culture medium. Meanwhile, the properties and structures of BC were characterized by Fourier Transform Infrared spec- troscopy, Scanning Electron Microscope and Tensile strength tester. The results indicated that the optimal medium for bacterial cellulose production were： 4.45 °Brix soybean molasses, 24.39 g/L nitrogen sources [peptone： yeast extract（m/m） =1：11, 1.42 g/L MgSO4, 1.95 g/L Na2HPO4 and 0.98 g/L citric acid. After fermentation for 5d in this medium, BC from soybean molasses was obtained at an yield of （5.85 ±0.27）g/L which was 2.15 times as high as that in glucose-based medium [（2.72 ±0.16） g/L]. In addition, there was no influence on the radical groups of BC obtained both in soybean molasses and glucose-based medium. The BC generated in soybean molasses had denser structure and its tensile strength increased significantly in comparison to the glucose-based medium during fermentation. At the same time, the water holding capacity was coincident （P〈0.05）.

关 键 词：大豆糖蜜 细菌纤维素 培养基优化 结构 性能 

分 类 号：O636.11[理学—高分子化学] TQ929[理学—化学]