鱼鳔类肝素提取工艺优化及抗凝血活性研究  

Optimization of Extraction process of heparinoid from swim bladder and evaluation its anticoagulant activity

作　　者：周斯仪 屈义 钟赛意[1,2,3] 杜振兴 苏伟明 谌素华[1] 洪鹏志 吴小禾[4] ZHOU Si-yi1, QU Yi1,ZHONG Sai-yi1,2,3, DU Zhen-xing1, SU Wei-min1,3 ,CHEN Su-hua1, HONG Peng-zhi1 ,WU Xiao-he4(1 Guangdong Ocean University, College of Food Science and Technology, Zhanjiang, Guangdong 524088, China 2. Shenzhen Research Institute of Guangdong Ocean University, Shenzhen, Guangdong 518108, China ; 3. Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Zhanjiang, Guangdong 524088, China;4. Zhongshan Torch Polytechnic, Zhongshan, Guangdong 528436, Chin)

机构地区：[1]广东海洋大学食品科技学院,广东湛江524088 [2]广东海洋大学深圳研究院,广东深圳518108 [3]广东省水产品加工与安全重点实验室,广东湛江524088 [4]中山火炬职业技术学院,广东中山528436

出　　处：《食品与机械》2018年第7期151-157,219,共8页Food and Machinery

基　　金：广东省渔港建设和渔业产业发展专项资金(编号:A201708C13);深圳市科技计划项目(编号:JCYJ20170818111335796);中山市淡水产品技术服务协同创新中心项目(编号:2016C1007);湛江市海洋经济创新发展示范市建设项目(编号:湛海创2017C8B1)

摘　　要：以鱼鳔为原料,采用酶法提取类肝素,研究其提取工艺参数和体外抗凝血活性。在单因素试验基础上,以加酶量、酶解时间和温度为影响因子,类肝素得率为响应值,采用响应曲面法优化鱼鳔类肝素(HSB)提取工艺,并通过测定活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)和血浆凝血酶时间(TT)评价其抗凝血活性。结果显示,最优提取条件为2709碱性蛋白酶添加量5.4 mg/mL,酶解温度50℃,时间20h,在该条件下类肝素得率为(1.79±0.05)%,与预测值相差3.24%;同时,所提取的类肝素具有一定的抗凝血活性,并通过外源性凝血途径和共同凝血途径发挥作用。A natural heparin-like compound was isolated from swim bladder and its anticoagulant activities were determined.The response surface analysis based on Box-Behnken design was adopted to examine effects of protease consumption,extracting time and temperature on heparin-like compound extraction.The anticoagulant activity of swim bladder heparin-like compound was evaluated by APTT,PT and TT method.Consequently,the optimum conditions were as follows.The concentration of protease from Bacillus licheniformis was 5.4 mg/mL,extracted at 50 ℃for 20 h.Under the control of these conditions, the predicted extraction rate by mathematical model was（1.79±0.05）%,and the standard error was3.24%.In addition,the heparin-like compound from swimming bladder（HSB）showed negligible in vitro anticoagulant activity,and played a role through the exogenous coagulation pathway and the common coagulation pathway.

关 键 词：鱼鳔 类肝素 酶法 抗凝血活性 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]