一种应用Asta-E-H脂肪酶定量检测雨生红球藻萃取物中虾青素的方法  

A method of detecting astaxanthin content in Haematococcus pluvialis extracted by lipase Asta-E-H

作　　者：高洁 宫平[1] 惠伯棣[1] 胡春凤 王旭 周牧函 杨楠 GAO Jie;GONG Ping;HUI Bo-di;HU Chun-feng;WANG Xu;ZHOU Mu-han;YANG Nan(Department of Food Science,Beijing Union University,Beijing 100023;Qinhuangdao Huien Biological Tech.Ltd.,Qinhuangdao 066004)

机构地区：[1]北京联合大学食品科学系,北京100023 [2]秦皇岛惠恩生物技术有限公司,秦皇岛066004

出　　处：《中国食品添加剂》2018年第10期93-102,共10页China Food Additives

摘　　要：目的:建立定量检测雨生红球藻及其萃取物中虾青素含量的方法。方法:使用新型脂肪酶AstaE-H催化水解虾青素酯,再采用外标法在C30-HPLC上对游离态虾青素进行定量。结果:测定结果的RSD值达到2.63%,加样回收率为100.08%,检测下限为40ng/mL,线性范围为0.04～25μg/mL。结论:应用AstaE-H脂肪酶的方法具有重复性好、灵敏度和回收率高、反应副产物(如虾红素和顺式异构体)少等优点,优于国标GB/T31520-2015方法,且成本远低于美国药典方法,可在相关产业中推广应用。Abstract ： Objective ： This study aims to develop a method for the quantification of astaxanthin content from Haematococcus pluvialis extracts. Method .. Astaxanthin esters from alga were hydrolyzed by a novel lipase catalysis of Asta-E-H. The astaxanthin was then quantified on C30-HPLC by external standard. Results ~ RSD of the method was 2.63% and the recovery rate was 100.08%. The detection limit was 40ng/mL, linear range was 0.04 - 25mg/ mL. Conclusion ~ The method with Asta-E-H has the advantages on satisfied reproducibility, high sensitivity and good recovery rate, and less by-products （eg. astacene and Z-isomer） compared with those of national standard GB/T31520-2015 ; the method costs less than U.S pharmacopoeia method. The method has potential to apply in the related fields.

关 键 词：雨生红球藻 虾青素酯 游离态虾青素 脂肪酶 水解 乳化 定量 

分 类 号：TS202.1[轻工技术与工程—食品科学] TS207.3[轻工技术与工程—食品科学与工程]