Low dose of resveratrol enhanced immune response of mice  

低剂量白藜芦醇增强小鼠免疫反应(英文)

作　　者：冯永红 周文亮 吴庆莉 李晓玉 赵维民 左建平 

机构地区：[1]中科院上海生命科学研究院药物研究所免疫药理室,上海中国200031

出　　处：《Acta Pharmacologica Sinica》2002年第10期31-35,共5页中国药理学报（英文版）

基　　金：Project supported by the One Hundred People Program of Chinese Academy of Sciences(1999),the National Natural Sci-ence Foundation of China,№ A30171086,and the Knowledge Innovation Program of Chinse Academy of Sciences,№KSCX2-SW-202.

摘　　要：AIM: To study the immune modulating effect of low dose of resveratrol. METHODS: Concanavalin A (ConA) and Staphylococcus aureus Cowan (Sac) were used to induce the activation of T lymphocyte and antigen presenting cell and cytokine production. [3H]-Thymidine incorporation was used to evaluate the proliferation of lymphocyte. Cytokine production was detected by ELISA method. Dinitrofluorobenzene (DNFB) was used to induce mice delayed type hypersensitivity (DTH, delayed hypersensitivity) response and ear swelling was used as an evaluating indicator. Changes of lymphocyte subtypes were detected by flow cytometry. RESULTS: Resveratrol (0.75-6 μmol/L) concentration-dependently promoted lymphocyte proliferation and IL-2 production induced by ConA. Sac induced IL-12 and IFN-γ (interferon type II) production were also concentration-dependently enhanced by resveratrol, while IL-10 production was inhibited. Resveratrol (4 mg/kg, ig) promoted DTH response of mouse, which was suppressed by ethanol (16 %, w/v) consumption. Resveratrol treatment had no significant influence on lymphocyte subtypes in mice, however it could reverse the suppressive effect of ethanol both on macrophage percentage and on macrophage MHC-II molecule expression. CONCLUSION: Low dose resveratrol enhanced cell-mediate immune response. Promoting Thl cytokine production and influencing on macrophage function might be its mechanisms.目的:研究低剂量白藜芦醇的免疫调节作用.方法:用ConA和Sac分别刺激T淋巴细胞和抗原细胞并诱导细胞因子产生;[~3H]-胸腺嘧啶核苷掺入法检测淋巴细胞增殖;ELISA法检测IL-2,IL-12,IL-10和IFN-γ;用DNFB诱导小鼠迟发型超敏反应(DTH),小鼠耳肿胀作为DTH反应指标;流式细胞仪检测小鼠脾淋巴细胞亚群的变化.结果:白藜芦醇(0.75-6μmol/L)剂量依赖性地促进小鼠T淋巴细胞的增殖和IL-2的产生;白藜芦醇还剂量依赖性地促进脾脏淋巴细胞IFN-γ和IL-12的生成,同时抑制IL-10的产生;白藜芦醇(4 mg/kg)灌胃给药能对抗乙醇(16％,w/v)对小鼠DTH反应的抑制作用;白藜芦醇对脾脏淋巴细胞亚群无明显改变,但能逆转乙醇对脾淋巴细胞中巨噬细胞数量和 MHC-II分子表达的下调作用.结论:低剂量白藜芦醇能促进小鼠细胞介导的免疫反应,促进Th1细胞因子产生及影响巨噬细胞功能是其可能的机制.

关 键 词：RESVERATROL T-LYMPHOCYTES INTERLEUKIN-12 INTERLEUKIN-10 interferon type II delayed hypersensitivity ethanol 

分 类 号：R285[医药卫生—中药学]