基于LC-MS/MS的酸乳肽谱及ACE抑制活性  

Peptide Profiling of Yogurts Identified by LC-MS/MS and Their ACE Inhibitory Activities

作　　者：于洋[1,2] 于文皓 晏嘉泽[2] 董浩[2] 董家耕 靳艳[2] 祁艳霞[1] YU Yang;YU Wenhao;YAN Jiaze;DONG Hao;DONG Jiageng;JIN Yan;QI Yanxia(College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China;Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical and Physics,Chinese Academy of Sciences, Dalian 116023, China;Liaoning Huishan Biotechnology Research Co. Ltd., Shenyang 110031, China)

机构地区：[1]大连海洋大学食品科学与工程学院,辽宁大连116023 [2]中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,辽宁大连116023 [3]辽宁辉山生物科技研究有限公司,辽宁沈阳110031

出　　处：《食品科学》2017年第5期208-212,共5页Food Science

基　　金：辽宁省博士启动基金项目(20141107)

摘　　要：利用液相色谱-串联质谱(liquid chromatography-tandem mass spectrometry,LC-MS/MS)的方法,对4种酸乳(优味舒风味发酵乳、风味发酵乳、初乳24+K风味发酵乳和十天风味发酵乳)的肽谱进行分析,分别鉴定到152、125、364条和274条肽段。利用血管紧张素转换酶(angiotensin converting enzyme,ACE)的抑制活性比较以上4种酸乳的生物活性,在相同质量浓度2.0 mg/L时,4种酸乳ACE抑制率分别为46.84%、42.75%、77.41%和63.84%。初乳24+K风味发酵乳含有肽的数量大、ACE抑制活性高可能与其独特的原料成分相关。对4种酸乳的肽谱及ACE抑制活性的考察,为进一步研究酸乳肽的功能特点和ACE抑制活性提供了理论依据。This study investigated the peptide profiling of four yogurt samples(Youweishu flavored fermented milk,plain flavored fermented milk,Churu24+K flavored fermented milk and Shitian flavored fermented milk)from local market by liquid chromatography-tandem mass spectrometry(LC-MS/MS).A total of152,125,364and274peptides were identified from the yogurts by LC-MS/MS,respectively.Angiotensin converting enzyme(ACE)inhibitory activity was used to evaluate the biological activities of yogurt peptides.The ACE inhibition rates of peptides from these yogurts were46.84%,42.75%,77.41%and63.84%at a concentration of2.0mg/L,respectively.The large number of identified peptides and high ACE inhibitory activities in Churu24+K flavor fermented milk may be related to its special ingredients.This study may provide the theoretical basis for further study on the functions and ACE inhibitory activity of yogurt peptides.

关 键 词：酸乳 肽谱 液相色谱-串联质谱 ACE抑制活性 

分 类 号：TS252.54[轻工技术与工程—农产品加工及贮藏工程]