酒曲中产凝乳酶微生物菌株的分离筛选及鉴定  

Isolation and Identification of Microbial Strains Producing Rennet from Jiuqu,a Traditional Chinese Fermentation Starter

作　　者：腾军伟 赵笑[1] 杨亚威[1] 张健[1] 赵爱梅[1] 姜云芸[1] 李柳[1] 郑喆[1] 杨贞耐[1] TENG Junwei;ZHAO Xiao;YANG Yawei;ZHANG Jian;ZHAO Aimei;JIANG Yunyun;LI Liu;ZHENG Zhe;YANG Zhennai(Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University (BTBU), Beijing 100048, China)

机构地区：[1]北京食品营养与人类健康高精尖创新中心,北京市食品添加剂工程技术研究中心,北京工商大学,北京100048

出　　处：《食品科学》2017年第16期23-28,共6页Food Science

基　　金：国家自然科学基金面上项目(31371804);国家自然科学基金青年科学基金项目(31601488);北京市百千万人才工程资助项目(B类);2016年北京工商大学研究生科研能力提升计划项目;公益性行业(农业)科研专项(201303085)

摘　　要：针对酒曲中的微生物进行分离纯化,得到11株细菌和2株真菌,并采用酪蛋白平板法和Arima时间法筛选出了1株产凝乳酶的细菌菌株编号为LB-51。通过形态学观察、生理生化实验和16S rDNA序列分析鉴定该菌株为解淀粉芽孢杆菌,将该产凝乳酶菌株命名为解淀粉芽孢杆菌GSBa-1。该菌株在液体LB培养基中发酵72 h产凝乳酶的凝乳活力为(431.53±15.89)SU/mL,蛋白水解活力为(5.05±0.59)U/mL,所产凝乳酶凝乳活力高而蛋白水解活力低,凝乳酶粗酶单位酶活力为1.54×10~5SU/g。解淀粉芽孢杆菌GSBa-1是分离筛选自酒曲中的一株高产凝乳酶细菌,因此其来源安全,可作为工业化候选菌株进一步研究开发。Eleven bacterial strains and two fungal strains were isolated from Jiuqu,a traditional Chinese fermentation starter for rice wine.Out of these,one bacterial strain,designated LB-51,capable of producing rennet was screened by the casein plate method and the Arima method.The strain was identified as Bacillus amyloliquefaciens by its morphological characteristics,physiological and biochemical tests(API50CHB system)and16S rDNA sequence analysis and species specific gene analysis and named as B.amyloliquefaciens GSBa-1.The rennet and proteolytic activities were(431.53±15.89)SU/mL and(5.05±0.59)U/mL,respectively,after72h shaking culture(120r/min)at30℃for72h in liquid LB medium.The enzyme exhibited high milk-clotting activity and low protein hydrolysis activity.The milk-clotting activity was1.54×105SU/g.B.amyloliquefaciens GSBa-1,an efficient producer and a safe source of rennet activity,is worth further research and development as a candidate strain for industrial production of rennet.

关 键 词：酒曲 凝乳酶 分离鉴定 解淀粉芽孢杆菌 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]