不同破壁方法对灵芝孢子粗多糖抗氧化活性的影响  

Effect of Different Cell Wall Disruption Methods on in Vitro Antioxidant Activity of Crude Polysaccharides Ganoderma lucidum Spores

作　　者：董扬[1,2] 郝利民[1,2] 刘宇琪[1,2] 张黎明[1] 鲁吉珂[2,3] 江庆伍 贾士儒[1] DONG Yang;HAO Limin;LIU Yuqi;ZHANG Liming;LU Jike;JIANG Qingwu;JIA Shiru(Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China;The Quartermaster Equipment Institute of People’s Liberation Army,Beijing 100010, China;School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China;Anhui Jinzhai Qiaokang Pharmaceutical Co. Ltd., Lu’an 237300, China)

机构地区：[1]天津科技大学工业发酵微生物教育部重点实验室,天津300457 [2]军需装备研究所,北京100010 [3]郑州大学生命科学学院,河南郑州450001 [4]安徽金寨乔康药业有限公司,安徽六安237300

出　　处：《食品科学》2017年第17期101-106,共6页Food Science

基　　金：全军重点科研项目(BX115C007;AX110C002);军需所科研项目(HX-04-14-025)

摘　　要：采用1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基清除法、羟自由基清除法、2,2’-联氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐自由基（2,2’-azinobis(3-ehtylbenzothiazolin-6-sulfnicacid)diammonium salt，ABTS＋·）清除法和铁氰化钾法以及酵母细胞氧化损伤保护模型，分别对未破壁灵芝孢子（unbroken Ganoderma lucidum spore，UGS）、机械法破壁灵芝孢子（mechanically broken Ganoderma lucidum spore，MGS）、生物法与机械法复合破壁的灵芝孢子（biologically and mechanically broken Ganoderma lucidum spore，BGS）提取粗多糖进行体外抗氧化活性评价。结果表明，3种处理方法的灵芝孢子粗多糖表现出不同的抗氧化活性，且抗氧化活性与多糖质量浓度呈一定效量关系，多糖质量浓度越高，抗氧化活性越强。BGS所得粗多糖对DPPH自由基、羟自由基、ABTS＋.的清除能力以及还原力在3种处理方法中均为最强。多糖质量浓度为5.0mg/mL时，对3种自由基的清除率分别为84.6%、91.0%、99.9%，还原力为1.09；BGS所得粗多糖对紫外损伤酵母细胞保护能力也相对较强。表明BGS粗多糖具有较高的抗氧化活性。The present work was undertaken to investigate the in vitro antioxidant activity of crude polysaccharides extracted from Ganoderma lucidum spores with unbroken(UGS)cell wall,and those with cell wall broken mechanically(MGS)and biologically-mechanically(BGS)in terms of their scavenging activities against DPPH,hydroxyl and ABTS radicals and K3Fe(CN)6reducing power and their ability to protect yeast cells from oxidative damage.The results showed that the three crude polysaccharides had different antioxidant activities in a dose-dependent manner.The scavenging capacities against DPPH,hydroxyl and ABTS radicals,and the reducing power of BGS polysaccharides were the highest among the three polysaccharides.At5.0mg/mL,the scavenging rates of DPPH,hydroxyl and ABTS radicals were84.6%,91.0%,and99.9%,respectively,and the reducing power was1.09.The protective effect of BGS polysaccharides on UV-induced oxidative damage was relatively strong.Therefore,the crude polysaccharide from Ganoderma lucidum spores with biologically and mechanically broken sporoderm has high antioxidant activity

关 键 词：灵芝孢子 生物破壁 机械破壁 粗多糖 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]