Expression of CXCL12-CXCR4 in osteosarcoma and its correlation with angiogenesis  

作　　者：Lu Han Yangyang Shen Wenhua Zhao Baoyong Sun Xin Zhang Kai Cui Lei Zhou Sheng Li 

机构地区：[1]School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250117, China [2]Department of Hepatobiliary Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China [3]Department of Anesthesiology, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China [4]Department of Tumor Minimally Invasive Surgery, Qianfo Hill Hospital of Shandong Province, Jinan 250014, China [5]Department of Bone & Soft Tissue Surgery, Shandong Academy of Medical Sciences, Shandong Cancer Hospital affiliated to Shandong University, Jinan 250117, China [6]Shandong Institute of Pharmaceutical Research, Jinan 250062, China [7]Shandong Provincial Collaborative Innovation Center for Neurodegenerative Disorders, Qingdao University, Qingdao 266071, China

出　　处：《Oncology and Translational Medicine》2017年第6期254-259,共6页肿瘤学与转化医学（英文版）

摘　　要：Objective The expression of CXCL12(stromal cell-derived factor-1)-CXCR4(chemokine receptors-4) in osteosarcoma and its role in angiogenesis were examined.Methods The expression of CXCR4 and CXCL12 in 40 cases of osteosarcoma was detected by immunohistochemistry and real-time fluorescence quantitative PCR.The expression of CD34 in osteosarcoma was detected by immunohistochemistry.Morphometric image analysis was performed to measure microvessel density(MVD).Additionally,the relationship between CXCL12 and CXCR4 expression and MVD of osteosarcoma and pulmonary metastasis were analyzed.Results The positive rates of CXCL12 and CXCR4 protein expression in osteosarcoma were 40.0%(16/40) and 60.0%(24/40),respectively.Fluorescence quantitative real-time PCR indicated that the expression level of CXCR4 m RNA in pulmonary metastatic osteosarcoma was higher than that in nonpulmonary metastatic osteosarcoma(P < 0.01).The level of MVD in pulmonary metastatic osteosarcoma was higher than that in non-pulmonary metastatic osteosarcoma(P < 0.01).Conclusion The expression level of CXCR4 was significantly associated with pulmonary metastasis and angiogenesis of osteosarcoma.Objective The expression of CXCL12(stromal cell-derived factor-1)-CXCR4(chemokine receptors-4) in osteosarcoma and its role in angiogenesis were examined.Methods The expression of CXCR4 and CXCL12 in 40 cases of osteosarcoma was detected by immunohistochemistry and real-time fluorescence quantitative PCR.The expression of CD34 in osteosarcoma was detected by immunohistochemistry.Morphometric image analysis was performed to measure microvessel density(MVD).Additionally,the relationship between CXCL12 and CXCR4 expression and MVD of osteosarcoma and pulmonary metastasis were analyzed.Results The positive rates of CXCL12 and CXCR4 protein expression in osteosarcoma were 40.0%(16/40) and 60.0%(24/40),respectively.Fluorescence quantitative real-time PCR indicated that the expression level of CXCR4 m RNA in pulmonary metastatic osteosarcoma was higher than that in nonpulmonary metastatic osteosarcoma(P < 0.01).The level of MVD in pulmonary metastatic osteosarcoma was higher than that in non-pulmonary metastatic osteosarcoma(P < 0.01).Conclusion The expression level of CXCR4 was significantly associated with pulmonary metastasis and angiogenesis of osteosarcoma.

关 键 词：OSTEOSARCOMA STROMAL cell-derived factor-1 (CXCL12) CHEMOKINE receptors-4 (CXCR4) ANGIOGENESIS pulmonary metastatics 

分 类 号：R738.1[医药卫生—肿瘤]