响应面法优化蛹虫草固态发酵产物虫草素与腺苷综合提取工艺  

Optimization of Simultaneous Extraction of Cordycepin and Adenosine Produced by Solid-State Fermentation with Cordyceps militaris by Response Surface Methodology

作　　者：于戈[1] 王丽[1] 胡欣蕾 孙亚男[1] 张欣[1] 李铭[1] 李文香[1] YU Ge;WANG Li;HU Xinlei;SUN Yanan;ZHANG Xin;LI Ming;LI Wenxiang(College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China)

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109

出　　处：《食品科学》2018年第6期270-275,共6页Food Science

基　　金：山东省现代农业产业技术体系建设经费资助项目(SDAIT-07-07);山东省科技发展计划项目(2014GNC113007)

摘　　要：以大米、啤酒糟共培养的蛹虫草固态发酵产物为原料,采用浸提法综合提取虫草素与腺苷,并利用响应面法优化提取条件。通过单因素试验考察提取时间、乙醇溶液体积分数、液料比和提取温度对综合提取虫草素与腺苷得率的影响。在单因素试验基础上,利用Box-Behnken试验设计,对浸提工艺中各影响因素进行优化。结果表明,浸提法综合提取虫草素与腺苷的最适工艺条件为纯水提取、提取温度45℃、液料比22∶1(m L/g)、提取时间87 min,在该条件下虫草素与腺苷的综合得率为2.491‰,与模型理论预测值相近,说明该模型回归性良好,试验的拟合程度高。蛹虫草固态发酵产物中富含虫草素、腺苷,具有一定开发价值。Cordycepin and adenosine were simultaneously extracted from the solid-state culture of Cordyceps militaris with rice and brewer’s spent grain(BSG)as co-substrates.The extraction process was optimized using one-factor-at-a time method and response surface methodology(RSM).The total yield of cordycepin and adenosine was investigated as a function of four variables including extraction time,ethanol concentration,solvent-to-solid ratio and temperature.The optimal extraction conditions were found to be extraction at45℃for87min with water as a solvent at a solvent-to-solid ratio of22:1(mL/g).Under these conditions,The maximum total yield(2.491‰)of cordycepin and adenosine was experimentally obtained,which agreed with the predicted value(2.487‰).Accordingly,the regression model developed showed excellent goodness of fit.The solid-state culture of Cordyceps militaris is a promising rich of cordycepin and adenosine.

关 键 词：蛹虫草 固态发酵产物 综合提取 响应面法 

分 类 号：S567.32[农业科学—中草药栽培]