乳源酪蛋白糖巨肽对结肠癌HT-29细胞抗炎及血管生成因子表达水平的影响  

Effect of Casein Glycomacropeptide(CGMP) on the Expression of Inflammatory Cytokines and Angiogenic Factors in HT-29 Cells

作　　者：王秋萍 贾彦 曹江鸣 赵培[1] 崔文静 马新颖 赵林森 闫亚丽[1] 陈庆森[1] WANG Qiuping;JIA Yan;CAO Jiangming;ZHAO Pei;CUI Wenjing;MA Xinying;ZHAO Linsen;YAN Yali;CHEN Qingsen(Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,Tianjin 300134, China;Hebei Inatural Biological Technical Company, Shijiazhuang 050899, China)

机构地区：[1]天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津300134 [2]河北一然生物科技有限公司,河北石家庄050899

出　　处：《食品科学》2018年第5期179-185,共7页Food Science

基　　金：国家自然科学基金面上项目(31071522)

摘　　要：研究乳源酪蛋白糖巨肽(casein glycomacropeptide,CGMP)对结肠癌细胞中的核转录因子(nucleartranscription factor,NF)-κB信号通路的作用,为其抗炎活性提供理论依据。本研究在前期探究"乳源酪蛋白糖巨肽对结肠癌HT-29细胞中COX-2、iNOS、GST-π表达的影响"的基础上,首先用脂多糖(lipopolysaccharide,LPS)刺激结肠癌HT-29细胞将NF-κB信号通路激活,采用免疫荧光法观测p65核移位来确定;随后用乳源CGMP干预激活NF-κB通路的HT-29细胞,用酶联免疫吸附剂测定细胞培养液中炎症因子和血管生成因子的表达水平,以探讨乳源CGMP消除结肠癌细胞活力的相关分子的变化。结果显示,1μg/mL的LPS能显著激活NF-κB信号通路,p65的核移位达到最佳水平;不同质量浓度的乳源CGMP均可不同程度地抑制HT-29细胞炎症因子和血管生成因子的表达水平。因此,乳源CGMP能够显著调节激活后的NF-κB信号通路,并有效抑制和改善结肠癌细胞的炎症状态,其机制为乳源CGMP通过有效抑制炎症因子和血管生成因子的表达,从而减缓结肠癌细胞的炎症程度。The effect of casein glycomacropeptide(CGMP)on the nuclear transcription factor(NF)-κB signaling pathway in colorectal carcinoma cells was assessed in order to provide a theoretical basis for its anti-inflammatory activity.As an extension of our previous work investigating the influence of CGMP on the expression of COX-2,iNOS and GST-πin colon cancer HT-29cells,this study determined whether the NF-κB signaling pathway in LPS challenged HT-29cells was activated through observing p65nuclear translocation by immunofluorescence microscopy.The challenged cells were treated with CGMP and the expression levels of inflammatory cytokines and angiogenesis factors in the cell cultures were detected by enzyme-linked immunosorbent assay(ELISA).The results showed that1μg/mL LPS significantly activated the NF-κB signaling pathway,and resulted in optimized nuclear translocation of p65.CGMP at all investigated concentrations could inhibit the expression levels of inflammatory factors and angiogenic factors in HT-29cells.Therefore,CGMP was capable of regulating the activated NF-κB signaling pathway and effectively restraining and improving the state of inflammation in colon cancer cells.This may be ascribed to inhibited expression of inflammatory cytokines and angiogenic factors,consequently reducing inflammation degree.

关 键 词：酪蛋白糖巨肽 结肠癌HT-29细胞 NF-ΚB 炎症因子 血管生成因子 

分 类 号：R151.2[医药卫生—营养与食品卫生学]