基于微信的“微生物遗传育种实验”混合式教学模式探究  

Research on the blended learning mode of “Microbial Breeding Experiments” based on WeChat

作　　者：严婷婷[1] 张蕾[1] 李余动[1] 梁新乐[1] Tingting Yan;Lei Zhang;Yudong Li;Xinle Liang(College of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China)

机构地区：[1]浙江工商大学食品与生物工程学院,杭州310018

出　　处：《遗传》2018年第7期607-612,共6页Hereditas（Beijing)

基　　金：国家自然科学基金面上项目(编号:31671836)资助~~

摘　　要：随着互联网的飞速发展,传统课堂教学与互联网相结合的混合式教学模式越来越受到人们的关注。微信作为使用最广泛的即时通讯软件,其公众号功能非常适合作为移动学习的平台。本文介绍了将微信应用到"微生物遗传育种实验"的教学实践,探索线上和线下结合的混合式教学模式。以"绿色荧光蛋白(green fluorescent protein,GFP)的基因定点突变实验"为例,从教学设计、建立公众号及推送素材、课前预习、课堂学习、课后复习及反馈等5个方面详细介绍混合式教学过程。GFP基因定点突变实验在引物上引入一个GFP突变位点(Y66H),以质粒p GFPuv为模板,经PCR扩增后,以DpnⅠ消化原始质粒,并转化大肠杆菌筛选蓝色荧光蛋白突变株。采用微信与课堂教学结合的模式,既方便学生与老师交流互动,又有利于学生利用碎片化时间学习,使得"教与学"更加顺畅。实践证明,这种混合式教学模式深受学生喜爱,增强了学生学习兴趣与学习自主性,显著提高了教学效果。With the rapid development of the internet technology,the blended learning mode plays a more and more important role in education reforms by integrating traditional classroom teaching and online learning.WeChat is the most popular Chinese social software,and its public platform is also suitable for mobile learning.Here,we report an application of the blended learning method based on WeChat public platforms in microbiology breeding experiment courses,using the site-directed mutagenesis of the green fluorescent protein(GFP)as an example.The learning process was divided into five modules:teaching design,learning resource preparation,pre-class learning,classroom learning,post-class review and evaluation.By introducing one mutation(Y66H)in mutagenic primers,the mutated GFP gene was amplified by PCR using pGFPuv as templates,followed by removal of the original plasmid template by DpnⅠdigestion.Students can monitor the color changes from green to blue in the fluorescence emission of the mutated proteins.As a useful addition to classroom teaching,WeChat is suitable for students to use fragmented time to learn and improve teaching interaction.Learning assessment results revealed the blended learning environment improves students’study interests and self-learning abilities,thus achieving a fruitful teaching result.

关 键 词：微信 混合式教学 基因突变 绿色荧光蛋白 

分 类 号：G434[文化科学—教育学] Q933-4[文化科学—教育技术学]