原花青素A2肠道菌群代谢物及其抗氧化活性  

Analysis of in Vitro Intestinal Microbial Metabolites of Procyanidin A2 and Evaluation of Their Antioxidant Activity

作　　者：杨光美[1,2] 李桐云 张玉莹 杨瑞丽 李武[1] YANG Guangmei;LI Tongyun;ZHANG Yuying;YANG Ruili;LI Wu(College of Food Science and Technology,Hainan University,Haikou 570228,China;Key Laboratory of Food Quality and Safety of Guangdong Province,College of Food Science,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]海南大学食品学院,海南海口570228 [2]广东省食品质量安全重点实验室,华南农业大学食品学院,广东广州510642

出　　处：《食品科学》2018年第21期155-159,共5页Food Science

基　　金：海南省自然科学基金项目(317007);国家自然科学基金面上项目(31471705);现代农业产业技术体系加工技术岗位专家项目(CARS-33)。

摘　　要：采用超高效液相色谱-四极杆-飞行时间质谱联用仪对原花青素A2(procyanidin A2,PCA2)体外大鼠肠道菌群代谢物进行分析,比较不同培养时间点PCA2肠道菌群代谢物总抗氧化能力(total antioxidant capacity,T-AOC)、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt,ABTS)自由基清除能力的变化。结果表明:随着菌群培养时间的延长,PCA2质量浓度逐渐降低,在6 h时PCA2的浓度降低77.28%,24 h降低了90.07%。PCA2经肠道菌群代谢主要产生8种新化合物,经鉴定为对羟苯基乙酸、表儿茶素、3-(4-羟苯基)丙酸、B型原花青素、5,7,2’-三羟基黄烷和3种未知结构化合物。PCA2肠道菌群代谢产物的抗氧化能力显著高于PCA2,其中代谢6 h时,其T-AOC、DPPH自由基和ABTS+μ清除能力分别提高了2.01、2.16、1.34倍。肠道菌群代谢物在PCA2生物活性作用过程中具有重要作用。The metabolites of procyanidin A2(PCA2)were analyzed using ultra performance liquid chromatographyquadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS)after incubation with the rat intestinal microbiota in vitro.The total antioxidant capacity(T-AOC)and 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)radical scavenging ability of PCA2 and its metabolites at different incubation times were measured and compared.PCA2 was degraded by 77.28%at 6 h and by 90.07%at 24 h of incubation.Eight metabolites were identified namely:4-hydroxyphenylacetic acid,epicatechin,3-(4-hydroxyphenyl)propionic acid,B type proanthocyanidins,5,7,2’-trihydroxyflavanone,and three unknown compounds.The antioxidant capacity of PCA2 was enhanced significantly after incubation.T-AOC,DPPH,ABTS radical scavenging ability after incubation for 6 h were significantly increased by 2.01,2.16 and 1.34 times,respectively compared with that before incubation.The results suggested that the activity of PCA2 might be partially attributed to its intestinal microbial metabolites.

关 键 词：原花青素A2 肠道菌群 代谢产物 抗氧化活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]