银杏花粉黄酮苷的恶味乳杆菌B2生物转化产物及其抗氧化活性评价  

Biotransformation of Ginkgo biloba Pollen Flavonoid Glycosides by Lacbacillus perolens B_2 and Evaluation of Antioxidant Activities

作　　者：裘纪莹 魏朝治 陈相艳[1] 杨金玉[1] 张翔[1] 陈蕾蕾[1] 王易芬[1] 李大鹏[2] QIU Jiying;WEI Chaozhi;CHEN Xiangyan;YANG Jinyu;ZHANG Xiang;CHEN Leilei;WANG Yifen;LI Dapeng(Key Laboratory of Agro-products Processing Technology of Shandong Province,Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture,Institute of Agro-food Science and Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,China;College of Food Science and Engineering,Shandong Agricultural University,Tai’an 271018,China)

机构地区：[1]山东省农业科学院农产品研究所,山东省农产品精深加工技术重点实验室,农业部新食品资源加工重点实验室,山东济南250100 [2]山东农业大学食品科学与工程学院,山东泰安271018

出　　处：《食品科学》2018年第21期203-209,共7页Food Science

基　　金：山东省泰山学者工程项目;山东省农业科学院创新工程项目(CXGC2017B06;CXGC2017A01);山东省自然科学基金项目(ZR2016YL022);山东省农业科学院青年英才培养计划项目。

摘　　要：目的:研究恶味乳杆菌B2(Lacbacillus perolens B2)生物转化对银杏花粉黄酮组分及其抗氧化活性的影响。方法:分析银杏花粉及其生物转化产物的提取物和各自石油醚相、乙酸乙酯相和正丁醇相萃取物的得率和高效液相色谱图,通过2,2’-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)、1,1-二苯基-2-三硝基苯肼自由基清除能力评价其体外抗氧化活性,通过对H_2O_2损伤小鼠巨噬细胞RAW264.7的保护作用评价其细胞抗氧化活性。结果:通过L. perolens B2的生物转化,提取物及各相萃取物得率均有所提高,银杏花粉主要黄酮苷被转化为以山柰酚为代表的黄酮苷元,并富集于乙酸乙酯萃取相。体外抗氧化活性评价显示,通过L. perolens B2的生物转化,提取物和各相萃取物的体外抗氧化能力均得到提高,其中生物转化产物的乙酸乙酯相萃取物的体外抗氧化活性最高。细胞实验显示,供试的银杏花粉及其生物转化产物的提取物和各自的乙酸乙酯相萃取物均可减缓由H_2O_2损伤导致的RAW264.7细胞凋亡,并且存在剂量依赖关系。生物转化产物的提取物及乙酸乙酯相萃取物对H_2O_2损伤RAW264.7细胞的保护能力均高于对应的银杏花粉样品,其中生物转化产物的乙酸乙酯相萃取物保护能力最强,当其质量浓度为15μg/mL时,几乎可以完全保护RAW264.7细胞不受H_2O_2损伤。结论:通过L. perolens B2的生物转化,银杏花粉主要黄酮苷被转化为以山柰酚为代表的黄酮苷元,提取物及各相萃取物的得率、体外抗氧化活性及对H_2O_2诱导损伤的RAW264.7细胞的保护作用均显著提高(P<0.05),其中生物转化产物的乙酸乙酯相萃取物抗氧化活性最高。Objective:This paper describes the effect of biotransformation by Lacbacillus perolens B2 on the flavonoid composition and antioxidant activities of Ginkgo biloba pollen.Methods:The yields and high performance liquid chromatography of the ethanol extracts of G.biloba pollen and its bioconverted products and their fractions in petroleum ether,ethyl acetate and n-butanol phases were analyzed,and their in vitro antioxidant activities were evaluated by 2-2’-azino-bis-(3-ethyl-benzthiazoline-6-sulfonic acid)and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities.By the protective effect on H2O2-induced injury in RAW264.7 mouse macrophages,their cellular antioxidant activities were also evaluated.Results:After bioconversion by L.perolens B2,the yields of all the extract and fractions were increased.The main flavonoid glycosides of G.biloba pollen were transformed into flavone aglycones such as kaempferol,enriched in the ethyl acetate phase.The in vitro antioxidant activities were improved as well,and the ethyl acetate extractable fraction of the biotransformation products showed the highest in vitro antioxidant activity.Cell experiments showed that the extracts of G.biloba pollen and its biotransformation products and their ethyl acetate soluble fractions could alleviate H2O2-induced apoptosis in RAW264.7 cells in a dose-dependent manner.The biotransformed extract and its ethyl acetate soluble fraction had stronger ability to protect RAW264.7 macrophages from H2O2-induced damage than their native counterparts.The biotransformed ethyl acetate soluble fraction had the highest protective ability,and it could completely protect RAW264.7 macrophages from H2O2-induced damage at a concentration of 15μg/mL.Conclusion:L.perolens B2 can biotransform the major flavonoid glycosides of G.biloba pollen into flavone aglycones such as kaempferol and significantly improve the yields,in vitro antioxidant activities and protection against H2O2-induced injury in RAW264.7 macrophages of the crude extract and its fractions in va

关 键 词：银杏花粉 恶味乳杆菌B2 黄酮 生物转化产物 体外抗氧化活性 细胞抗氧化活性 

分 类 号：TS201.4[轻工技术与工程—食品科学]