Alcalase蛋白酶对草鱼鱼鳞胶原多肽提取的研究  

Study on the Extraction of Collagen Polypeptide from Grass Carp Fish by Alcalase protease

作　　者：赵莎 Zhao Sha(Xi'an Pei hua University,Shaanxi Xi'an 710125)

机构地区：[1]西安培华学院,陕西西安710125

出　　处：《生物化工》2019年第1期103-105,共3页Biological Chemical Engineering

基　　金：西安培华学院2018年度校级科研项目(PHKT18064)

摘　　要：本实验以草鱼鱼鳞作为原料,采用Alcalase蛋白酶对其进行水解并提取草鱼鱼鳞胶原多肽。通过对胶原多肽提取条件进行探究,最佳提取条件为:烘干加热处理温度121℃,处理时间10 min、加酶量0.07 AU/g、酶解时间3 h、酶解温度60℃、底物质量分数15%。实验结果表明:在此条件下,草鱼鱼鳞胶原多肽的提取率较高。In this experiment,grass carp scales were used as raw materials,and alcalase protease was used to hydrolyze and extract grass carp scale collagen peptides.Through the exploration and optimization of collagen peptide extraction conditions,the experimental results are as follows:drying and heating treatment temperature is 121℃,treatment time is 10 min,enzyme dosage is 0.07 AU/g,enzymatic hydrolysis time is 3 h,enzymatic hydrolysis temperature is 60℃,and the score of substrate quality is 15%.The experimental results show that under these conditions,the extraction rate of grass carp fish scale collagen peptide is higher.

关 键 词：草鱼 鱼鳞 胶原多肽 ALCALASE蛋白酶 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]